{"doi":"10.1038/ncomms12456","title":"The myosin X motor is optimized for movement on actin bundles","abstract":"<jats:title>Abstract</jats:title><jats:p>Myosin X has features not found in other myosins. Its structure must underlie its unique ability to generate filopodia, which are essential for neuritogenesis, wound healing, cancer metastasis and some pathogenic infections. By determining high-resolution structures of key components of this motor, and characterizing the <jats:italic>in vitro</jats:italic> behaviour of the native dimer, we identify the features that explain the myosin X dimer behaviour. Single-molecule studies demonstrate that a native myosin X dimer moves on actin bundles with higher velocities and takes larger steps than on single actin filaments. The largest steps on actin bundles are larger than previously reported for artificially dimerized myosin X constructs or any other myosin. Our model and kinetic data explain why these large steps and high velocities can only occur on bundled filaments. Thus, myosin X functions as an antiparallel dimer in cells with a unique geometry optimized for movement on actin bundles.</jats:p>","journal":"Nature Communications","year":2016,"id":623602,"datarank":2.508087471563872,"base_score":4.59511985013459,"endowment":4.59511985013459,"self_citation_contribution":0.6892679775201885,"citation_network_contribution":1.8188194940436835,"self_endowment_contribution":0.6892679775201885,"citer_contribution":1.8188194940436835,"corpus_percentile":null,"corpus_rank":null,"citation_count":98,"citer_count":79,"citers_with_citation_signal":72,"citers_with_endowment":72,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":20937,"name":"Zhaohui Yang","orcid":"0000-0003-0958-4439","position":1,"is_corresponding":false},{"id":1611676,"name":"Tatiana Isabet","orcid":null,"position":2,"is_corresponding":false},{"id":1611678,"name":"Florian Blanc","orcid":null,"position":3,"is_corresponding":false},{"id":579518,"name":"Kaifeng Zhou","orcid":"0000-0003-4428-3447","position":4,"is_corresponding":false},{"id":1122938,"name":"Tianming Lin","orcid":null,"position":5,"is_corresponding":false},{"id":1235973,"name":"Xiaoyan Liu","orcid":"0000-0003-1905-1740","position":6,"is_corresponding":false},{"id":1611679,"name":"Pascale Hissier","orcid":null,"position":7,"is_corresponding":false},{"id":1611680,"name":"Frédéric Samazan","orcid":null,"position":8,"is_corresponding":false},{"id":1611681,"name":"Béatrice Amigues","orcid":null,"position":9,"is_corresponding":false},{"id":1611682,"name":"Eric D. Yang","orcid":null,"position":10,"is_corresponding":false},{"id":517790,"name":"Hyokeun Park","orcid":"0000-0002-2655-4795","position":11,"is_corresponding":false},{"id":688373,"name":"Olena Pylypenko","orcid":"0000-0001-7073-5238","position":12,"is_corresponding":false},{"id":1204584,"name":"Marco Cecchini","orcid":"0000-0003-2671-1583","position":13,"is_corresponding":false},{"id":297371,"name":"Charles V. Sindelar","orcid":"0000-0002-6646-7776","position":14,"is_corresponding":false},{"id":7872,"name":"H. Lee Sweeney","orcid":"0000-0002-6290-8853","position":15,"is_corresponding":false},{"id":353410,"name":"Anne Houdusse","orcid":"0000-0002-8566-0336","position":16,"is_corresponding":false},{"id":1212481,"name":"Virginie Ropars","orcid":"0000-0002-3372-6030","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"The myosin X motor is optimized for movement on actin bundles","abstract":"<jats:title>Abstract</jats:title><jats:p>Myosin X has features not found in other myosins. Its structure must underlie its unique ability to generate filopodia, which are essential for neuritogenesis, wound healing, cancer metastasis and some pathogenic infections. By determining high-resolution structures of key components of this motor, and characterizing the <jats:italic>in vitro</jats:italic> behaviour of the native dimer, we identify the features that explain the myosin X dimer behaviour. Single-molecule studies demonstrate that a native myosin X dimer moves on actin bundles with higher velocities and takes larger steps than on single actin filaments. The largest steps on actin bundles are larger than previously reported for artificially dimerized myosin X constructs or any other myosin. Our model and kinetic data explain why these large steps and high velocities can only occur on bundled filaments. Thus, myosin X functions as an antiparallel dimer in cells with a unique geometry optimized for movement on actin bundles.</jats:p>","is_dataset_classified":null,"base_score":4.59511985013459,"endowment":4.59511985013459,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"27580874","pmcid":"PMC5025751","openalex_id":"https://openalex.org/W2508255303","authors":[],"funders":[{"funder_name":"NIDCD NIH HHS","grant_id":"R01 DC009100","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM110530","title":null},{"funder_name":"NHLBI NIH HHS","grant_id":"P01 HL110869","title":null},{"funder_name":"French National Research Agency (ANR)","grant_id":"ANR-13-BSV8-0019","title":"Cellular functions of Myosin V and Myosin X – Role of partners and specific design"},{"funder_name":"French National Research Agency (ANR)","grant_id":"ANR-10-IDEX-0001","title":null},{"funder_name":"National Institutes of Health","grant_id":"5P01HL110869-03","title":"Myosin Mutations Leading to Vascular Diseases"}],"total_grants":6,"fwci":3.533,"citation_percentile":0.9369958,"influential_citations":0,"citation_trend":[{"year":2016,"count":1},{"year":2017,"count":8},{"year":2018,"count":5},{"year":2019,"count":3},{"year":2020,"count":14},{"year":2021,"count":15},{"year":2022,"count":10},{"year":2023,"count":17},{"year":2024,"count":15},{"year":2025,"count":7},{"year":2026,"count":3}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://www.nature.com/articles/ncomms12456.pdf","host_type":"journal"},{"url":"https://www.nature.com/articles/ncomms12456.pdf","host_type":"publisher"},{"url":"https://www.nature.com/articles/ncomms12456","host_type":"publisher"},{"url":"https://doi.org/10.1038/ncomms12456","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/27580874","host_type":"repository"},{"url":"https://hal.sorbonne-universite.fr/hal-01361523","host_type":"repository"},{"url":"https://doaj.org/article/82998216bea24bff892439a8a874030a","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/5025751","host_type":"repository"},{"url":"http://www.scopus.com/record/display.url?eid=2-s2.0-84985994987&origin=inward","host_type":"repository"},{"url":"http://lbsearch.ust.hk:3210/sfx?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rfr_id=info:sid/HKUST:SPI&rft.genre=article&rft.issn=2041-1723&rft.volume=&rft.issue=&rft.date=2016&rft.spage=&rft.aulast=Ropars&rft.aufirst=V.&rft.atitle=The+myosin+X+motor+is+optimized+for+movement+on+actin+bundles&rft.title=Nature+Communications","host_type":"repository"},{"url":"http://www.nature.com/articles/ncomms12456","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC5025751","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC5025751?pdf=render","host_type":"Europe_PMC"},{"url":"http://dx.doi.org/10.1038/ncomms12456","host_type":""},{"url":"https://dx.doi.org/10.1038/ncomms12456","host_type":""},{"url":"https://hal.sorbonne-universite.fr/hal-01361523v1/document","host_type":""},{"url":"https://hal.sorbonne-universite.fr/hal-01361523v1","host_type":""},{"url":"https://doi.org/https://doi.org/10.1038/ncomms12456","host_type":""}],"fields_of_study":["Cardiomyopathy and Myosin Studies","Cellular Mechanics and Interactions","Muscle Physiology and Disorders","0301 basic medicine","0303 health sciences","03 medical and health sciences","Actin Cytoskeleton","Animals","Cattle","Cell Membrane","Crystallography, X-Ray","Dimerization","Humans","Molecular Docking Simulation","Molecular Dynamics Simulation","Myosins","Pseudopodia"],"mesh_terms":["Animals","Cattle","Cell Membrane","Humans","Actin Cytoskeleton","Myosins","Pseudopodia","Crystallography, X-Ray","Dimerization","Molecular Dynamics Simulation","Molecular Docking Simulation"],"keywords":["Myosin","Filopodia","Antiparallel (mathematics)","Actin","Dimer","Molecular motor","Myosin head","Microfilament","Biophysics","Motor protein","Chemistry","Myosin light-chain kinase","Biology","Cell biology","Cytoskeleton","Physics","Biochemistry","Microtubule","Science","Q","Cell Membrane","Molecular Dynamics Simulation","Myosins","Crystallography, X-Ray","Article","Molecular Docking Simulation","Actin Cytoskeleton","[SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology","Animals","Humans","Cattle","Pseudopodia","Dimerization"],"sdg_mappings":[{"sdg_number":3,"sdg_label":"3. Good health"},{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"emdb"},{"name":"pdb"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-04T00:31:41.162996Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}