{"doi":"10.1038/ncomms1225","title":"Errors in the measurement of voltage-activated ion channels in cell-attached patch-clamp recordings","abstract":null,"journal":"Nature Communications","year":2011,"id":602138,"datarank":0.515098080672772,"base_score":3.4339872044851463,"endowment":3.4339872044851463,"self_citation_contribution":0.515098080672772,"citation_network_contribution":0.0,"self_endowment_contribution":0.515098080672772,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":30,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1539701,"name":"Christian Wozny","orcid":null,"position":1,"is_corresponding":false},{"id":614751,"name":"Stephen R. Williams","orcid":"0000-0003-2108-2757","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Errors in the measurement of voltage-activated ion channels in cell-attached patch-clamp recordings","abstract":"Patch-clamp recording techniques have revolutionized understanding of the function and sub-cellular location of ion channels in excitable cells. The cell-attached patch-clamp configuration represents the method of choice to describe the endogenous properties of voltage-activated ion channels in the axonal, somatic and dendritic membrane of neurons, without disturbance of the intracellular milieu. Here, we directly examine the errors associated with cell-attached patch-clamp measurement of ensemble ion channel activity. We find for a number of classes of voltage-activated channels, recorded from the soma and dendrites of neurons in acute brain-slices and isolated cells, that the amplitude and kinetics of ensemble ion channel activity recorded in cell-attached patches is significantly distorted by transmembrane voltage changes generated by the flow of current through the activated ion channels. We outline simple error-correction procedures that allow a more accurate description of the density and properties of voltage-activated channels to be incorporated into computational models of neurons.","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"21097893","pmcid":null,"openalex_id":null,"authors":[],"funders":[{"funder_name":"European Commission","grant_id":"235256","title":"Information processing in distal dendrites of neocortical layer 5 pyramidal neurons"}],"total_grants":1,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":"hybrid","license":"cc-by-nc-sa","oa_locations":[{"url":"https://www.nature.com/articles/ncomms1225.pdf","host_type":"publisher"},{"url":"https://www.nature.com/articles/ncomms1225","host_type":"publisher"},{"url":"https://doi.org/10.1038/ncomms1225","host_type":""},{"url":"https://pubmed.ncbi.nlm.nih.gov/21407208","host_type":""},{"url":"http://dx.doi.org/10.1038/ncomms1225","host_type":""},{"url":"https://strathprints.strath.ac.uk/60705/1/Williams_Wozny_NC_2011_Errors_in_the_measurement_of_voltage_activated_ion_channels.pdf","host_type":""},{"url":"https://api.library.uq.edu.au/view/UQ:258135","host_type":""},{"url":"https://dx.doi.org/10.1038/ncomms1225","host_type":""},{"url":"https://strathprints.strath.ac.uk/60705/","host_type":""},{"url":"https://doi.org/https://doi.org/10.1038/ncomms1225","host_type":""}],"fields_of_study":["0301 basic medicine","03 medical and health sciences","0303 health sciences"],"mesh_terms":[],"keywords":["571","Patch-Clamp Techniques","1300 Biochemistry","610","Cyclic Nucleotide-Gated Cation Channels","Gene Expression","Genetics and Molecular Biology","Models, Biological","Article","Membrane Potentials","In-vitro","QH301","Mice","Electricity","1300 Biochemistry, Genetics and Molecular Biology","Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels","Animals","Humans","3100 Physics and Astronomy","Synaptic potentials","Biology","Action-potential backpropagation","Membrane-properties","Ions","Neurons","Cell Membrane","Electric Conductivity","Brain","Dendrites","1600 Chemistry","H-channels","HEK293 Cells","Pacemaker channels","Rat","Ca1 pyramidal neurons","K+ channels","Artifacts","Ion Channel Gating","Plasmids"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-29T18:26:35.698453Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}