{"doi":"10.1038/nchembio872","title":"The Cfd1–Nbp35 complex acts as a scaffold for iron-sulfur protein assembly in the yeast cytosol","abstract":null,"journal":"Nature Chemical Biology","year":2007,"id":678540,"datarank":0.7747178960885273,"base_score":5.1647859739235145,"endowment":5.1647859739235145,"self_citation_contribution":0.7747178960885273,"citation_network_contribution":0.0,"self_endowment_contribution":0.7747178960885273,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":174,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1772878,"name":"Antonio J Pierik","orcid":null,"position":1,"is_corresponding":false},{"id":1734632,"name":"Martin Stümpfig","orcid":null,"position":2,"is_corresponding":false},{"id":1605289,"name":"Ulrich Mühlenhoff","orcid":null,"position":3,"is_corresponding":false},{"id":1171473,"name":"Roland Lill","orcid":"0000-0002-8345-6518","position":4,"is_corresponding":false},{"id":1772877,"name":"Daili J A Netz","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"The Cfd1–Nbp35 complex acts as a scaffold for iron-sulfur protein assembly in the yeast cytosol","abstract":"Biogenesis of iron-sulfur ([Fe-S]) proteins in eukaryotes requires the function of complex proteinaceous machineries in both mitochondria and cytosol. In contrast to the mitochondrial pathway, little is known about [Fe-S] protein assembly in the cytosol. So far, four highly conserved proteins (Cfd1, Nbp35, Nar1 and Cia1) have been identified as members of the cytosolic [Fe-S] protein assembly machinery, but their molecular function is unresolved. Using in vivo and in vitro approaches, we found that the soluble P-loop NTPases Cfd1 and Nbp35 form a complex and bind up to three [4Fe-4S] clusters, one at the N terminus of Nbp35 and one each at a new C-terminal cysteine-rich motif present in both proteins. These labile [Fe-S] clusters can be rapidly transferred and incorporated into target [Fe-S] apoproteins in a Nar1- and Cia1-dependent fashion. Our data suggest that the Cfd1-Nbp35 complex functions as a novel scaffold for [Fe-S] cluster assembly in the eukaryotic cytosol.","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"17401378","pmcid":null,"openalex_id":null,"authors":[],"funders":[],"total_grants":0,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":"closed","license":"http://www.springer.com/tdm","oa_locations":[{"url":"http://www.nature.com/articles/nchembio872.pdf","host_type":"publisher"},{"url":"http://www.nature.com/articles/nchembio872","host_type":"publisher"}],"fields_of_study":["Adenosine Triphosphatases","Apoproteins","Cytosol","Enzyme Activation","GTP-Binding Proteins","Iron-Sulfur Proteins","Isomerases","Protein Binding","Protein Processing, Post-Translational","Saccharomyces cerevisiae","Saccharomyces cerevisiae Proteins"],"mesh_terms":["Cytosol","Saccharomyces cerevisiae","GTP-Binding Proteins","Isomerases","Apoproteins","Iron-Sulfur Proteins","Saccharomyces cerevisiae Proteins","Protein Processing, Post-Translational","Enzyme Activation","Protein Binding","Adenosine Triphosphatases"],"keywords":[],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-17T05:33:30.299002Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}