{"doi":"10.1038/ncb3188","title":"Microtubule-associated proteins control the kinetics of microtubule nucleation","abstract":null,"journal":"Nature Cell Biology","year":2015,"id":647662,"datarank":8.605130685675702,"base_score":5.53338948872752,"endowment":5.53338948872752,"self_citation_contribution":0.8300084233091282,"citation_network_contribution":7.7751222623665734,"self_endowment_contribution":0.8300084233091282,"citer_contribution":7.7751222623665734,"corpus_percentile":null,"corpus_rank":null,"citation_count":252,"citer_count":200,"citers_with_citation_signal":200,"citers_with_endowment":200,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":891484,"name":"Susanne Bechstedt","orcid":"0000-0002-4706-9975","position":1,"is_corresponding":false},{"id":1176851,"name":"Sami Chaaban","orcid":"0000-0003-1133-800X","position":2,"is_corresponding":false},{"id":556388,"name":"Gary J. Brouhard","orcid":"0000-0001-9101-1247","position":3,"is_corresponding":false},{"id":1687519,"name":"Michal Wieczorek","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Microtubule-associated proteins control the kinetics of microtubule nucleation","abstract":"Microtubules are born and reborn continuously, even during quiescence. These polymers are nucleated from templates, namely γ-tubulin ring complexes (γ-TuRCs) and severed microtubule ends. Using single-molecule biophysics, we show that nucleation from γ-TuRCs, axonemes and seed microtubules requires tubulin concentrations that lie well above the critical concentration. We measured considerable time lags between the arrival of tubulin and the onset of steady-state elongation. Microtubule-associated proteins (MAPs) alter these time lags. Catastrophe factors (MCAK and EB1) inhibited nucleation, whereas a polymerase (XMAP215) and an anti-catastrophe factor (TPX2) promoted nucleation. We observed similar phenomena in cells. We conclude that GTP hydrolysis inhibits microtubule nucleation by destabilizing the nascent plus ends required for persistent elongation. Our results explain how MAPs establish the spatial and temporal profile of microtubule nucleation.","is_dataset_classified":null,"base_score":5.53338948872752,"endowment":5.53338948872752,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"26098575","pmcid":null,"openalex_id":"https://openalex.org/W1555174091","authors":[],"funders":[{"funder_name":"Canadian Institutes of Health Research","grant_id":"MOP-111265","title":null},{"funder_name":"Natural Sciences and Engineering Research Council of Canada","grant_id":"unidentified","title":"unidentified"}],"total_grants":2,"fwci":12.0937,"citation_percentile":0.99263942,"influential_citations":0,"citation_trend":[{"year":2015,"count":6},{"year":2016,"count":15},{"year":2017,"count":26},{"year":2018,"count":36},{"year":2019,"count":26},{"year":2020,"count":34},{"year":2021,"count":28},{"year":2022,"count":19},{"year":2023,"count":15},{"year":2024,"count":27},{"year":2025,"count":12},{"year":2026,"count":8}],"oa_status":"closed","license":"Springer TDM","oa_locations":[{"url":"http://www.nature.com/articles/ncb3188.pdf","host_type":"publisher"},{"url":"http://www.nature.com/articles/ncb3188","host_type":"publisher"},{"url":"https://doi.org/10.1038/ncb3188","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/26098575","host_type":"repository"},{"url":"https://dx.doi.org/10.1038/ncb3188","host_type":""}],"fields_of_study":["Microtubule and mitosis dynamics","Ubiquitin and proteasome pathways","Genomics and Chromatin Dynamics","0301 basic medicine","0303 health sciences","03 medical and health sciences","Animals","Axoneme","CHO Cells","Cell Line, Tumor","Centrosome","Cricetinae","Cricetulus","Green Fluorescent Proteins","Guanosine Triphosphate","Humans","Hydrolysis","Immunoblotting","Kinetics","LLC-PK1 Cells","Microscopy, Electron","Microscopy, Fluorescence","Microtubule-Associated Proteins","Microtubules","Nocodazole","Polymerization","Swine","Tubulin","Tubulin Modulators"],"mesh_terms":["Animals","Cricetulus","Guanosine Triphosphate","Cricetinae","Humans","Hydrolysis","Kinetics","Microscopy, Electron","Microscopy, Fluorescence","Microtubule-Associated Proteins","Microtubules","Swine","Tubulin","Immunoblotting","Nocodazole","CHO Cells","LLC-PK1 Cells","Centrosome","Cell Line, Tumor","Green Fluorescent Proteins","Tubulin Modulators","Axoneme","Polymerization"],"keywords":["Microtubule","Nucleation","Microtubule nucleation","Tubulin","Elongation","Biophysics","Kinetics","Cell biology","Chemistry","Microtubule-associated protein","Biology","Physics","Materials science","Biochemistry","Cell","Centrosome","Cell cycle","Axoneme","Hydrolysis","Nocodazole","Green Fluorescent Proteins","Immunoblotting","CHO Cells","Microtubules","Polymerization","Microscopy, Electron","Cricetulus","Microscopy, Fluorescence","Cell Line, Tumor","Cricetinae","Animals","Humans","LLC-PK1 Cells","Guanosine Triphosphate","Microtubule-Associated Proteins"],"sdg_mappings":[{"sdg_number":3,"sdg_label":"3. 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