{"doi":"10.1038/ncb2702","title":"Kdm2b maintains murine embryonic stem cell status by recruiting PRC1 complex to CpG islands of developmental genes","abstract":null,"journal":"Nature Cell Biology","year":2013,"id":621115,"datarank":0.8703177563065596,"base_score":5.802118375377063,"endowment":5.802118375377063,"self_citation_contribution":0.8703177563065596,"citation_network_contribution":0.0,"self_endowment_contribution":0.8703177563065596,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":330,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1449191,"name":"Li Shen","orcid":"0009-0004-2377-5391","position":1,"is_corresponding":false},{"id":334884,"name":"Ma Wan","orcid":"0000-0002-4215-2614","position":2,"is_corresponding":false},{"id":283496,"name":"Olena Taranova","orcid":null,"position":3,"is_corresponding":false},{"id":780678,"name":"Hao Wu","orcid":"0000-0001-9222-2521","position":4,"is_corresponding":false},{"id":537977,"name":"Yi Zhang","orcid":"0000-0003-1780-0963","position":5,"is_corresponding":false},{"id":635001,"name":"Jin He","orcid":"0000-0002-5108-4182","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Kdm2b maintains murine embryonic stem cell status by recruiting PRC1 complex to CpG islands of developmental genes","abstract":"Polycomb group (PcG) proteins play important roles in repressing lineage-specific genes and maintaining the undifferentiated state of mouse embryonic stem cells (mESCs). However, how PcG proteins are recruited to their target genes is largely unknown. Here, we show that the H3K36-specific histone demethylase Kdm2b is highly expressed in mESCs and regulated by the pluripotent factors Oct4 and Sox2 directly. Depletion of Kdm2b in mESCs causes de-repression of lineage-specific genes and induces early differentiation. The function of Kdm2b depends on its CxxC-ZF domain, which mediates its genome-wide binding to CpG islands (CGIs). Kdm2b interacts with the core components of polycomb repressive complex 1 (PRC1) and recruits the complex to the CGIs of early lineage-specific genes. Thus, our study not only reveals an Oct4-Sox2-Kdm2b-PRC1-CGI regulatory axis and its function in maintaining the undifferentiated state of mESCs, but also demonstrates a critical function of Kdm2b in recruiting PRC1 to the CGIs of lineage-specific genes to repress their expression.","is_dataset_classified":null,"base_score":5.802118375377063,"endowment":5.802118375377063,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"23502314","pmcid":"PMC4078788","openalex_id":"https://openalex.org/W2009166526","authors":[],"funders":[{"funder_name":"NIDDK NIH HHS","grant_id":"U01DK089565","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"GM068804","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM068804","title":null},{"funder_name":"NIDDK NIH HHS","grant_id":"T32 DK060445","title":null},{"funder_name":"National Institutes of Health","grant_id":"5U01DK089565-05","title":"Epigenetic and chemical approaches to generate function beta cells"},{"funder_name":"Howard Hughes Medical Institute","grant_id":"","title":null},{"funder_name":"Howard Hughes Medical Institute","grant_id":"","title":null}],"total_grants":7,"fwci":14.3132,"citation_percentile":0.99403915,"influential_citations":0,"citation_trend":[{"year":2013,"count":12},{"year":2014,"count":31},{"year":2015,"count":33},{"year":2016,"count":28},{"year":2017,"count":29},{"year":2018,"count":26},{"year":2019,"count":28},{"year":2020,"count":30},{"year":2021,"count":31},{"year":2022,"count":31},{"year":2023,"count":19},{"year":2024,"count":11},{"year":2025,"count":13},{"year":2026,"count":8}],"oa_status":"green","license":"Springer TDM","oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4078788","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4078788","host_type":"repository"},{"url":"http://www.nature.com/articles/ncb2702.pdf","host_type":"publisher"},{"url":"http://www.nature.com/articles/ncb2702","host_type":"publisher"},{"url":"https://doi.org/10.1038/ncb2702","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/23502314","host_type":"repository"},{"url":"https://europepmc.org/articles/pmc4078788?pdf=render","host_type":""},{"url":"https://dx.doi.org/10.1038/ncb2702","host_type":""}],"fields_of_study":["Epigenetics and DNA Methylation","Genomics and Chromatin Dynamics","Pluripotent Stem Cells Research","Animals","Base Sequence","Biomarkers, Tumor","Blotting, Western","Cell Differentiation","Cell Lineage","Cell Proliferation","Cells, Cultured","Chromatin Immunoprecipitation","CpG Islands","DNA Methylation","Electrophoretic Mobility Shift Assay","Embryonic Stem Cells","F-Box Proteins","Fluorescent Antibody Technique","Gene Expression Profiling","Gene Expression Regulation, Developmental","Genes, Developmental","Genome","Immunoprecipitation","Jumonji Domain-Containing Histone Demethylases","Luciferases","Mice","Molecular Sequence Data","Octamer Transcription Factor-3","Oligonucleotide Array Sequence Analysis","Pluripotent Stem Cells","Polycomb Repressive Complex 1","Promoter Regions, Genetic","RNA, Messenger","RNA, Small Interfering","Real-Time Polymerase Chain Reaction","Repressor Proteins","Reverse Transcriptase Polymerase Chain Reaction","SOXB1 Transcription Factors","Sequence Homology, Nucleic Acid","Transfection"],"mesh_terms":["Animals","Base Sequence","Cell Differentiation","Cells, Cultured","Fluorescent Antibody Technique","Luciferases","Molecular Sequence Data","Promoter Regions, Genetic","Repressor Proteins","RNA, Messenger","Sequence Homology, Nucleic Acid","Transfection","Biomarkers, Tumor","Blotting, Western","Genome","Gene Expression Regulation, Developmental","CpG Islands","Cell Lineage","DNA Methylation","Reverse Transcriptase Polymerase Chain Reaction","Oligonucleotide Array Sequence Analysis","Gene Expression Profiling","Electrophoretic Mobility Shift Assay","RNA, Small Interfering","Pluripotent Stem Cells","F-Box Proteins","Chromatin Immunoprecipitation","Immunoprecipitation","Cell Proliferation","Genes, Developmental","Octamer Transcription Factor-3","Mice","Embryonic Stem Cells","SOXB1 Transcription Factors","Jumonji Domain-Containing Histone Demethylases","Real-Time Polymerase Chain Reaction","Polycomb Repressive Complex 1"],"keywords":["Demethylase","Polycomb-group proteins","Embryonic stem cell","Biology","SOX2","Lineage (genetic)","Histone","PRC2","Cell biology","Gene","Genetics","Histone H3","Repressor","Transcription factor","Chromatin Immunoprecipitation","Genome","Base Sequence","F-Box Proteins","Gene Expression Profiling","Blotting, Western","Fluorescent Antibody Technique","Gene Expression Regulation, Developmental","Cell Differentiation","Electrophoretic Mobility Shift Assay","DNA Methylation","Biomarkers, Tumor","Animals","Immunoprecipitation","Cell Lineage","CpG Islands","Genes, Developmental","Cells, Cultured","Embryonic Stem Cells","Cell Proliferation"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life below water"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"geo"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-03T13:16:02.202358Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}