{"doi":"10.1038/nbt.3658","title":"Targeted DNA demethylation in vivo using dCas9–peptide repeat and scFv–TET1 catalytic domain fusions","abstract":null,"journal":"Nature Biotechnology","year":2016,"id":592009,"datarank":10.54770873778,"base_score":6.2422232654551655,"endowment":6.2422232654551655,"self_citation_contribution":0.9363334898182749,"citation_network_contribution":9.611375247961726,"self_endowment_contribution":0.9363334898182749,"citer_contribution":9.611375247961726,"corpus_percentile":null,"corpus_rank":null,"citation_count":513,"citer_count":200,"citers_with_citation_signal":200,"citers_with_endowment":200,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":582289,"name":"Hirofumi Noguchi","orcid":"0000-0002-9779-4956","position":1,"is_corresponding":false},{"id":1514790,"name":"Takuro Horii","orcid":null,"position":2,"is_corresponding":false},{"id":1110708,"name":"Kazuhiko Nakabayashi","orcid":"0000-0003-2927-0963","position":3,"is_corresponding":false},{"id":1514792,"name":"Mika Kimura","orcid":null,"position":4,"is_corresponding":false},{"id":1110709,"name":"K. Okamura","orcid":"0000-0001-9747-5069","position":5,"is_corresponding":false},{"id":1514794,"name":"Atsuhiko Sakai","orcid":null,"position":6,"is_corresponding":false},{"id":1243170,"name":"Hideyuki Nakashima","orcid":"0000-0001-9430-5363","position":7,"is_corresponding":false},{"id":1133133,"name":"Kenichiro Hata","orcid":"0000-0002-7809-7637","position":8,"is_corresponding":false},{"id":1243172,"name":"Kinichi Nakashima","orcid":"0000-0001-5901-9628","position":9,"is_corresponding":false},{"id":473094,"name":"Izuho Hatada","orcid":"0000-0001-7225-3929","position":10,"is_corresponding":false},{"id":1514787,"name":"Sumiyo Morita","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Targeted DNA demethylation in vivo using dCas9–peptide repeat and scFv–TET1 catalytic domain fusions","abstract":"Despite the importance of DNA methylation in health and disease, technologies to readily manipulate methylation of specific sequences for functional analysis and therapeutic purposes are lacking. Here we adapt the previously described dCas9-SunTag for efficient, targeted demethylation of specific DNA loci. The original SunTag consists of ten copies of the GCN4 peptide separated by 5-amino-acid linkers. To achieve efficient recruitment of an anti-GCN4 scFv fused to the ten-eleven (TET) 1 hydroxylase, which induces demethylation, we changed the linker length to 22 amino acids. The system attains demethylation efficiencies >50% in seven out of nine loci tested. Four of these seven loci showed demethylation of >90%. We demonstrate targeted demethylation of CpGs in regulatory regions and demethylation-dependent 1.7- to 50-fold upregulation of associated genes both in cell culture (embryonic stem cells, cancer cell lines, primary neural precursor cells) and in vivo in mouse fetuses.","is_dataset_classified":null,"base_score":6.2422232654551655,"endowment":6.2422232654551655,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"27571369","pmcid":null,"openalex_id":"https://openalex.org/W2517955921","authors":[],"funders":[],"total_grants":0,"fwci":27.4129,"citation_percentile":0.99759084,"influential_citations":0,"citation_trend":[{"year":2016,"count":4},{"year":2017,"count":43},{"year":2018,"count":63},{"year":2019,"count":61},{"year":2020,"count":64},{"year":2021,"count":53},{"year":2022,"count":60},{"year":2023,"count":60},{"year":2024,"count":50},{"year":2025,"count":40},{"year":2026,"count":15}],"oa_status":"closed","license":"http://www.springer.com/tdm","oa_locations":[{"url":"http://www.nature.com/articles/nbt.3658.pdf","host_type":"publisher"},{"url":"http://www.nature.com/articles/nbt.3658","host_type":"publisher"},{"url":"https://doi.org/10.1038/nbt.3658","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/27571369","host_type":"repository"}],"fields_of_study":["Epigenetics and DNA Methylation","Genetics and Neurodevelopmental Disorders","RNA modifications and cancer"],"mesh_terms":["Protein Domains","Animals","Catalysis","Genetic Engineering","Mixed Function Oxygenases","Proto-Oncogene Proteins","Gene Targeting","CpG Islands","DNA Methylation","Saccharomyces cerevisiae Proteins","Basic-Leucine Zipper Transcription Factors","Mice","Single-Chain Antibodies","Clustered Regularly Interspaced Short Palindromic Repeats","CRISPR-Associated Proteins"],"keywords":["DNA demethylation","Demethylation","DNA methylation","Methylation","Biology","In vivo","DNA","Embryonic stem cell","Molecular biology","Peptide","Gene","Cell biology","Chemistry","Genetics","Biochemistry","Gene expression"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-26T11:47:20.820491Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}