{"doi":"10.1038/nbt.1523","title":"Solution hybrid selection with ultra-long oligonucleotides for massively parallel targeted sequencing","abstract":"Targeting genomic loci by massively parallel sequencing requires new methods to enrich templates to be sequenced. We developed a capture method that uses biotinylated RNA 'baits' to fish targets out of a 'pond' of DNA fragments. The RNA is transcribed from PCR-amplified oligodeoxynucleotides originally synthesized on a microarray, generating sufficient bait for multiple captures at concentrations high enough to drive the hybridization. We tested this method with 170-mer baits that target >15,000 coding exons (2.5 Mb) and four regions (1.7 Mb total) using Illumina sequencing as read-out. About 90% of uniquely aligning bases fell on or near bait sequence; up to 50% lay on exons proper. The uniformity was such that approximately 60% of target bases in the exonic 'catch', and approximately 80% in the regional catch, had at least half the mean coverage. One lane of Illumina sequence was sufficient to call high-confidence genotypes for 89% of the targeted exon space.","journal":"Nature Biotechnology","year":2009,"id":12435,"datarank":1.0972827584629463,"base_score":7.315218389752975,"endowment":7.315218389752975,"self_citation_contribution":1.0972827584629463,"citation_network_contribution":0.0,"self_endowment_contribution":1.0972827584629463,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1502,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.0476,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2009-02-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":16797,"name":"Alexandre Melnikov","orcid":"0000-0001-5346-1773","position":1,"is_corresponding":false},{"id":60356,"name":"Jared Maguire","orcid":null,"position":2,"is_corresponding":false},{"id":16801,"name":"Peter Rogov","orcid":"0000-0002-1578-7251","position":3,"is_corresponding":false},{"id":16524,"name":"Emily M LeProust","orcid":null,"position":4,"is_corresponding":false},{"id":98048,"name":"William Brockman","orcid":null,"position":5,"is_corresponding":false},{"id":995,"name":"Anne H. O’Donnell-Luria","orcid":"0000-0001-6418-9592","position":6,"is_corresponding":false},{"id":29088,"name":"Georgia Giannoukos","orcid":null,"position":7,"is_corresponding":false},{"id":25084,"name":"Sheila Fisher","orcid":"0000-0002-8681-2362","position":8,"is_corresponding":false},{"id":7676,"name":"Carsten Russ","orcid":null,"position":9,"is_corresponding":false},{"id":27305,"name":"Anthony Philippakis","orcid":"0000-0001-6953-3794","position":10,"is_corresponding":false},{"id":20075,"name":"David B. Jaffe","orcid":"0000-0001-8739-568X","position":11,"is_corresponding":false},{"id":18887,"name":"Matthew E. Hurles","orcid":"0000-0002-2333-7015","position":12,"is_corresponding":false},{"id":1745,"name":"Irene Newsham","orcid":"0000-0003-4913-298X","position":13,"is_corresponding":false},{"id":98063,"name":"William W. Brockman","orcid":null,"position":14,"is_corresponding":false},{"id":63162,"name":"Timothy R. Fennell","orcid":"0000-0001-5632-0159","position":15,"is_corresponding":false},{"id":7055,"name":"Andreas Gnirke","orcid":"0000-0001-5324-6387","position":0,"is_corresponding":true}],"reference_count":25,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-03-01T18:20:47.508186Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}