{"doi":"10.1038/nature17970","title":"Near-atomic resolution visualization of human transcription promoter opening","abstract":null,"journal":"Nature","year":2016,"id":589656,"datarank":9.267690561366296,"base_score":5.726847747587197,"endowment":5.726847747587197,"self_citation_contribution":0.8590271621380796,"citation_network_contribution":8.408663399228216,"self_endowment_contribution":0.8590271621380796,"citer_contribution":8.408663399228216,"corpus_percentile":null,"corpus_rank":null,"citation_count":306,"citer_count":200,"citers_with_citation_signal":200,"citers_with_endowment":200,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":424476,"name":"Chunli Yan","orcid":"0000-0001-5742-120X","position":1,"is_corresponding":false},{"id":381374,"name":"Jie Fang","orcid":"0000-0002-6962-4183","position":2,"is_corresponding":false},{"id":574827,"name":"Carla Inouye","orcid":"0000-0002-9216-1383","position":3,"is_corresponding":false},{"id":109375,"name":"Robert Tjian","orcid":"0000-0003-0539-8217","position":4,"is_corresponding":false},{"id":308121,"name":"Ivaylo Ivanov","orcid":"0000-0002-5306-1005","position":5,"is_corresponding":false},{"id":308992,"name":"Eva Nogales","orcid":"0000-0001-9816-3681","position":6,"is_corresponding":false},{"id":1403201,"name":"Yuan He","orcid":"0000-0002-6196-2276","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Near-atomic resolution visualization of human transcription promoter opening","abstract":"In eukaryotic transcription initiation, a large multi-subunit pre-initiation complex (PIC) that assembles at the core promoter is required for the opening of the duplex DNA and identification of the start site for transcription by RNA polymerase II. Here we use cryo-electron microscropy (cryo-EM) to determine near-atomic resolution structures of the human PIC in a closed state (engaged with duplex DNA), an open state (engaged with a transcription bubble), and an initially transcribing complex (containing six base pairs of DNA-RNA hybrid). Our studies provide structures for previously uncharacterized components of the PIC, such as TFIIE and TFIIH, and segments of TFIIA, TFIIB and TFIIF. Comparison of the different structures reveals the sequential conformational changes that accompany the transition from each state to the next throughout the transcription initiation process. This analysis illustrates the key role of TFIIB in transcription bubble stabilization and provides strong structural support for a translocase activity of XPB.","is_dataset_classified":null,"base_score":5.726847747587197,"endowment":5.726847747587197,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"27193682","pmcid":"PMC4940141","openalex_id":"https://openalex.org/W2358564002","authors":[],"funders":[{"funder_name":"NIGMS NIH HHS","grant_id":"GM110387","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM110387","title":null},{"funder_name":"NCI NIH HHS","grant_id":"P30 CA060553","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"GM63072","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM063072","title":null},{"funder_name":"National Institutes of Health","grant_id":"1R01GM110387-01A1","title":"Integrative modeling of PCNA assemblies engaged in genome duplication and repair"},{"funder_name":"National Science Foundation","grant_id":"1149521","title":"CAREER: Modeling Assemblies and Interactions at the Replication Fork: Sliding Clamps and Clamp Loaders"},{"funder_name":"Howard Hughes Medical Institute","grant_id":"","title":null}],"total_grants":8,"fwci":22.283,"citation_percentile":0.99660889,"influential_citations":0,"citation_trend":[{"year":2016,"count":20},{"year":2017,"count":45},{"year":2018,"count":41},{"year":2019,"count":32},{"year":2020,"count":37},{"year":2021,"count":38},{"year":2022,"count":21},{"year":2023,"count":20},{"year":2024,"count":22},{"year":2025,"count":19},{"year":2026,"count":11}],"oa_status":"closed","license":"IUCr Copyright and Licensing Policy","oa_locations":[{"url":"https://www.nature.com/articles/nature17970.pdf","host_type":"publisher"},{"url":"https://www.nature.com/articles/nature17970","host_type":"publisher"},{"url":"https://doi.org/10.1038/nature17970","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/27193682","host_type":"repository"},{"url":"https://escholarship.org/uc/item/00p826rv","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4940141","host_type":"repository"},{"url":"http://www.escholarship.org/uc/item/00p826rv","host_type":"repository"},{"url":"https://doi.org/10.1107/s0108767317097483","host_type":""},{"url":"http://journals.iucr.org/a/issues/2017/a1/00/a54901/a54901.pdf","host_type":""},{"url":"https://europepmc.org/articles/pmc4940141?pdf=render","host_type":""},{"url":"https://dx.doi.org/10.1038/nature17970","host_type":""},{"url":"https://dx.doi.org/10.1107/s0108767317097483","host_type":""},{"url":"https://doi.org/https://doi.org/10.1038/nature17970","host_type":""}],"fields_of_study":["RNA and protein synthesis mechanisms","RNA modifications and cancer","Advanced Electron Microscopy Techniques and Applications","0301 basic medicine","0303 health sciences","03 medical and health sciences"],"mesh_terms":["DNA","DNA Helicases","DNA-Binding Proteins","HeLa Cells","Humans","Models, Molecular","Movement","Promoter Regions, Genetic","Protein Conformation","RNA Polymerase II","Saccharomyces cerevisiae","Transcription Factors, TFII","Cryoelectron Microscopy","Multiprotein Complexes","Transcription Initiation, Genetic","Transcription Elongation, Genetic","Hela Cells"],"keywords":["Transcription factor II A","Transcription factor II F","Transcription factor II H","Transcription factor II B","Transcription factor II E","General transcription factor","RNA polymerase II","RNA polymerase II holoenzyme","Transcription preinitiation complex","Transcription (linguistics)","Eukaryotic transcription","Transcription bubble","Transcription factor II D","Biology","Cell biology","Promoter","RNA polymerase","Genetics","Molecular biology","RNA","Gene expression","Gene","Models, Molecular","DNA-Binding Proteins (mesh)","Transcription Elongation, Genetic","Protein Conformation","General Science & Technology (science-metrix)","TFII (mesh)","Transcription Factors, TFII","Models","Hela Cells (mesh)","Promoter Regions, Genetic","Transcription Initiation, Genetic","Cryoelectron Microscopy (mesh)","Humans (mesh)","51 Physical Sciences (for-2020)","Generic health relevance (hrcs-hc)","Biological Sciences","DNA-Binding Proteins","Molecular (mesh)","Multiprotein Complexes (mesh)","Physical Sciences","DNA Helicases (mesh)","Transcription Initiation","General Science & Technology","1.1 Normal biological development and functioning","Movement","610","Saccharomyces cerevisiae","Promoter Regions","Genetic","Humans","Saccharomyces cerevisiae (mesh)","1.1 Normal biological development and functioning (hrcs-rac)","TFII","31 Biological Sciences (for-2020)","Protein Conformation (mesh)","Genetics (rcdc)","Cryoelectron Microscopy","RNA Polymerase II (mesh)","DNA Helicases","Molecular","DNA","Movement (mesh)","540","3101 Biochemistry and Cell Biology (for-2020)","Multiprotein Complexes","Genetic (mesh)","Biochemistry and Cell Biology","Generic health relevance","DNA (mesh)","Transcription Elongation","Transcription Factors","HeLa Cells"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"emdb"},{"name":"pdb"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-24T01:58:47.488328Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}