{"doi":"10.1038/nature11648","title":"The TEL patch of telomere protein TPP1 mediates telomerase recruitment and processivity","abstract":null,"journal":"Nature","year":2012,"id":47441,"datarank":10.136002661479424,"base_score":5.8805329864007,"endowment":5.8805329864007,"self_citation_contribution":0.8820799479601051,"citation_network_contribution":9.253922713519318,"self_endowment_contribution":0.8820799479601051,"citer_contribution":9.253922713519318,"corpus_percentile":null,"corpus_rank":null,"citation_count":357,"citer_count":200,"citers_with_citation_signal":200,"citers_with_endowment":200,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":219603,"name":"Caitlin F. Bell","orcid":null,"position":1,"is_corresponding":false},{"id":219604,"name":"Ina Weidenfeld","orcid":null,"position":2,"is_corresponding":false},{"id":218797,"name":"Arthur J. Zaug","orcid":null,"position":3,"is_corresponding":false},{"id":219605,"name":"Leslie A. Leinwand","orcid":null,"position":4,"is_corresponding":false},{"id":218800,"name":"Thomas R. Cech","orcid":null,"position":5,"is_corresponding":false},{"id":218799,"name":"Jayakrishnan Nandakumar","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"The TEL patch of telomere protein TPP1 mediates telomerase recruitment and processivity","abstract":"Human chromosome ends are capped by shelterin, a protein complex that protects the natural ends from being recognized as sites of DNA damage and also regulates the telomere-replicating enzyme, telomerase. Shelterin includes the heterodimeric POT1-TPP1 protein, which binds the telomeric single-stranded DNA tail. TPP1 has been implicated both in recruiting telomerase to telomeres and in stimulating telomerase processivity (the addition of multiple DNA repeats after a single primer-binding event). Determining the mechanisms of these activities has been difficult, especially because genetic perturbations also tend to affect the essential chromosome end-protection function of TPP1 (refs 15-17). Here we identify separation-of-function mutants of human TPP1 that retain full telomere-capping function in vitro and in vivo, yet are defective in binding human telomerase. The seven separation-of-function mutations map to a patch of amino acids on the surface of TPP1, the TEL patch, that both recruits telomerase to telomeres and promotes high-processivity DNA synthesis, indicating that these two activities are manifestations of the same molecular interaction. Given that the interaction between telomerase and TPP1 is required for telomerase function in vivo, the TEL patch of TPP1 provides a new target for anticancer drug development.","is_dataset_classified":null,"base_score":5.8805329864007,"endowment":5.8805329864007,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"23103865","pmcid":"PMC3521872","openalex_id":"https://openalex.org/W2030594866","authors":[],"funders":[{"funder_name":"NCI NIH HHS","grant_id":"K99 CA167644","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R01GM29090","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R01GM099705","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM029090","title":null},{"funder_name":"National Institutes of Health","grant_id":"5R01GM099705-06","title":"TERT Promoter Mutations and Telomerase Reactivation in Cancer Cells"},{"funder_name":"National Institutes of Health","grant_id":"2R01GM029090-30A1","title":"Myosin Gene Diversity and Function"},{"funder_name":"National Institutes of Health","grant_id":"1K99CA167644-01A1","title":"Separation-of-function Mutants to Study the Biological Significance of Telomerase"},{"funder_name":"Howard Hughes Medical Institute","grant_id":"","title":null},{"funder_name":"Howard Hughes Medical Institute","grant_id":"","title":null}],"total_grants":9,"fwci":14.2486,"citation_percentile":0.99442164,"influential_citations":35,"citation_trend":[{"year":2012,"count":1},{"year":2013,"count":23},{"year":2014,"count":38},{"year":2015,"count":28},{"year":2016,"count":30},{"year":2017,"count":23},{"year":2018,"count":27},{"year":2019,"count":26},{"year":2020,"count":27},{"year":2021,"count":29},{"year":2022,"count":28},{"year":2023,"count":29},{"year":2024,"count":26},{"year":2025,"count":11},{"year":2026,"count":10}],"oa_status":"closed","license":"Springer TDM","oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3521872","host_type":"GREEN"},{"url":"http://www.nature.com/articles/nature11648.pdf","host_type":"publisher"},{"url":"http://www.nature.com/articles/nature11648","host_type":"publisher"},{"url":"https://doi.org/10.1038/nature11648","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/23103865","host_type":"repository"},{"url":"https://www.nature.com/articles/nature11648","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3521872","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC3521872","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC3521872?pdf=render","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/pmc3521872?pdf=render","host_type":""},{"url":"http://dx.doi.org/10.1038/nature11648","host_type":""},{"url":"https://dx.doi.org/10.1038/nature11648","host_type":""}],"fields_of_study":["Telomeres, Telomerase, and Senescence","DNA Repair Mechanisms","Advanced biosensing and bioanalysis techniques","Biology","Medicine","0301 basic medicine","0303 health sciences","03 medical and health sciences","Cell Line","HEK293 Cells","HeLa Cells","Humans","Models, Molecular","Mutation","Protein Binding","Protein Structure, Tertiary","Shelterin Complex","Telomerase","Telomere","Telomere-Binding Proteins"],"mesh_terms":["Shelterin Complex","Cell Line","HeLa Cells","Humans","Models, Molecular","Mutation","Protein Binding","Telomere","Protein Structure, Tertiary","Telomerase","Telomere-Binding Proteins","HEK293 Cells","Hela Cells"],"keywords":["Shelterin","Telomerase","Telomere","Processivity","Telomere-binding protein","Biology","DNA","Cell biology","Molecular biology","Primer (cosmetics)","Genetics","DNA replication","DNA-binding protein","Chemistry","Gene","Transcription factor","Models, Molecular","Telomere-Binding Proteins","Article","Shelterin Complex","Cell Line","Protein Structure, Tertiary","HEK293 Cells","Mutation","Humans","HeLa Cells","Protein Binding"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"pdb"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-18T17:12:14.575798Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}