{"doi":"10.1038/msb4100050","title":"Construction of\n                    <i>Escherichia coli</i>\n                    K‐12 in‐frame, single‐gene knockout mutants: the Keio collection","abstract":"We have systematically made a set of precisely defined, single-gene deletions of all nonessential genes in Escherichia coli K-12. Open-reading frame coding regions were replaced with a kanamycin cassette flanked by FLP recognition target sites by using a one-step method for inactivation of chromosomal genes and primers designed to create in-frame deletions upon excision of the resistance cassette. Of 4288 genes targeted, mutants were obtained for 3985. To alleviate problems encountered in high-throughput studies, two independent mutants were saved for every deleted gene. These mutants-the 'Keio collection'-provide a new resource not only for systematic analyses of unknown gene functions and gene regulatory networks but also for genome-wide testing of mutational effects in a common strain background, E. coli K-12 BW25113. We were unable to disrupt 303 genes, including 37 of unknown function, which are candidates for essential genes. Distribution is being handled via GenoBase (http://ecoli.aist-nara.ac.jp/).","journal":"Molecular Systems Biology","year":2006,"id":2384,"datarank":11.105539417323516,"base_score":9.020147920801659,"endowment":9.020147920801659,"self_citation_contribution":1.353022188120249,"citation_network_contribution":9.752517229203267,"self_endowment_contribution":1.353022188120249,"citer_contribution":9.752517229203267,"corpus_percentile":99.14133209561383,"corpus_rank":112,"citation_count":8267,"citer_count":100,"citers_with_citation_signal":100,"citers_with_endowment":100,"datacite_reuse_total":0,"is_dataset":true,"is_dataset_confidence":0.5407,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2006-01-01","fair_score":77.9167,"fair_percentile":89.0,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":29258,"name":"Takeshi Ara","orcid":"0000-0003-1754-2837","position":1,"is_corresponding":false},{"id":29259,"name":"Miki Hasegawa","orcid":"0000-0003-0082-2576","position":2,"is_corresponding":false},{"id":29260,"name":"Yuki Takai","orcid":"0000-0001-6506-5313","position":3,"is_corresponding":false},{"id":29261,"name":"Yoshiko Okumura","orcid":null,"position":4,"is_corresponding":false},{"id":29262,"name":"Miki Baba","orcid":null,"position":5,"is_corresponding":false},{"id":29263,"name":"Kirill A. 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