{"doi":"10.1038/jcbfm.2009.55","title":"Patterns and Dynamics of Subventricular Zone Neuroblast Migration in the Ischemic Striatum of the Adult Mouse","abstract":"<jats:p>The migratory behavior of neuroblasts after a stroke is poorly understood. Using time-lapse microscopy, we imaged migration of neuroblasts and cerebral vessels in living brain slices of adult doublecortin (DCX, a marker of neuroblasts) enhanced green fluorescent protein (eGFP) transgenic mice that were subjected to 7 days of stroke. Our results show that neuroblasts originating in the subventricular zone (SVZ) of adult mouse brain laterally migrated in chains or individually to reach the ischemic striatum. The chains were initially formed at the border between the SVZ and the striatum by neuroblasts in the SVZ and then extended to the striatum. The average speed of DCX-eGFP-expressing cells within chains was 28.67 ± 1.04 μm/h, which was significantly faster ( P &lt; 0.01) than the speed of the cells in the SVZ (17.98 ± 0.57 μm/h). Within the ischemic striatum, individual neuroblasts actively extended or retracted their processes, suggestive of probing the immediate microenvironment. The neuroblasts close to cerebral blood vessels exhibited multiple processes. Our data suggest that neuroblasts actively interact with the microenvironment to reach the ischemic striatum by multiple migratory routes.</jats:p>","journal":"Journal of Cerebral Blood Flow &amp; Metabolism","year":2009,"id":97947,"datarank":3.619561546703475,"base_score":4.762173934797756,"endowment":4.762173934797756,"self_citation_contribution":0.7143260902196635,"citation_network_contribution":2.9052354564838114,"self_endowment_contribution":0.7143260902196635,"citer_contribution":2.9052354564838114,"corpus_percentile":null,"corpus_rank":null,"citation_count":116,"citer_count":98,"citers_with_citation_signal":86,"citers_with_endowment":86,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":337609,"name":"Michael Chopp","orcid":"0000-0002-1948-4493","position":1,"is_corresponding":false},{"id":484186,"name":"Sara R Gregg","orcid":null,"position":2,"is_corresponding":false},{"id":484187,"name":"Yier Toh","orcid":null,"position":3,"is_corresponding":false},{"id":484188,"name":"Cindi Roberts","orcid":null,"position":4,"is_corresponding":false},{"id":484189,"name":"Yvonne LeTourneau","orcid":null,"position":5,"is_corresponding":false},{"id":464541,"name":"Benjamin Buller","orcid":null,"position":6,"is_corresponding":false},{"id":233250,"name":"Longfei Jia","orcid":"0009-0004-1852-5857","position":7,"is_corresponding":false},{"id":484190,"name":"Siamak P Nejad Davarani","orcid":null,"position":8,"is_corresponding":false},{"id":484191,"name":"Zheng G Zhang","orcid":null,"position":9,"is_corresponding":false},{"id":484185,"name":"Rui L Zhang","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Patterns and Dynamics of Subventricular Zone Neuroblast Migration in the Ischemic Striatum of the Adult Mouse","abstract":"<jats:p>The migratory behavior of neuroblasts after a stroke is poorly understood. Using time-lapse microscopy, we imaged migration of neuroblasts and cerebral vessels in living brain slices of adult doublecortin (DCX, a marker of neuroblasts) enhanced green fluorescent protein (eGFP) transgenic mice that were subjected to 7 days of stroke. Our results show that neuroblasts originating in the subventricular zone (SVZ) of adult mouse brain laterally migrated in chains or individually to reach the ischemic striatum. The chains were initially formed at the border between the SVZ and the striatum by neuroblasts in the SVZ and then extended to the striatum. The average speed of DCX-eGFP-expressing cells within chains was 28.67 ± 1.04 μm/h, which was significantly faster ( P &lt; 0.01) than the speed of the cells in the SVZ (17.98 ± 0.57 μm/h). Within the ischemic striatum, individual neuroblasts actively extended or retracted their processes, suggestive of probing the immediate microenvironment. The neuroblasts close to cerebral blood vessels exhibited multiple processes. Our data suggest that neuroblasts actively interact with the microenvironment to reach the ischemic striatum by multiple migratory routes.</jats:p>","is_dataset_classified":null,"base_score":4.762173934797756,"endowment":4.762173934797756,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"19436318","pmcid":"PMC2741163","openalex_id":"https://openalex.org/W2027022086","authors":[],"funders":[{"funder_name":"NINDS NIH HHS","grant_id":"P50 NS23392","title":null},{"funder_name":"NHLBI NIH HHS","grant_id":"R01HL 64766","title":null},{"funder_name":"NINDS NIH HHS","grant_id":"P01 NS042345","title":null},{"funder_name":"NINDS NIH HHS","grant_id":"P01 NS42345","title":null},{"funder_name":"NHLBI NIH HHS","grant_id":"R01 HL064766","title":null},{"funder_name":"NINDS NIH HHS","grant_id":"P50 NS023393","title":null}],"total_grants":6,"fwci":2.7441,"citation_percentile":0.88895743,"influential_citations":3,"citation_trend":[{"year":2012,"count":11},{"year":2013,"count":11},{"year":2014,"count":12},{"year":2015,"count":15},{"year":2016,"count":9},{"year":2017,"count":9},{"year":2018,"count":9},{"year":2019,"count":5},{"year":2020,"count":6},{"year":2021,"count":5},{"year":2022,"count":3},{"year":2023,"count":4},{"year":2024,"count":1},{"year":2025,"count":4},{"year":2026,"count":1}],"oa_status":"bronze","license":"https://journals.sagepub.com/page/policies/text-and-data-mining-license","oa_locations":[{"url":"https://journals.sagepub.com/doi/pdf/10.1038/jcbfm.2009.55","host_type":"journal"},{"url":"https://journals.sagepub.com/doi/pdf/10.1038/jcbfm.2009.55","host_type":"BRONZE"},{"url":"https://journals.sagepub.com/doi/pdf/10.1038/jcbfm.2009.55","host_type":"publisher"},{"url":"https://journals.sagepub.com/doi/full-xml/10.1038/jcbfm.2009.55","host_type":"publisher"},{"url":"https://doi.org/10.1038/jcbfm.2009.55","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/19436318","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2741163","host_type":"repository"}],"fields_of_study":["Neurogenesis and neuroplasticity mechanisms","Neuroinflammation and Neurodegeneration Mechanisms","Axon Guidance and Neuronal Signaling","Medicine","Biology","Animals","Brain Ischemia","Cell Movement","Cerebral Ventricles","Cerebrovascular Circulation","Corpus Striatum","Doublecortin Protein","Kinetics","Mice","Microscopy, Video","Neurons","Stem Cells","Stroke"],"mesh_terms":["Doublecortin Protein","Animals","Cell Movement","Brain Ischemia","Cerebral Ventricles","Cerebrovascular Circulation","Corpus Striatum","Kinetics","Neurons","Stem Cells","Microscopy, Video","Stroke","Mice"],"keywords":["Neuroblast","Subventricular zone","Doublecortin","Striatum","Neuroscience","Neurogenesis","Biology","Neural stem cell","Rostral migratory stream","Genetically modified mouse","Cell biology","Anatomy","Transgene","Central nervous system","Dopamine","Stem cell"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-18T22:36:25.439953Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}