{"doi":"10.1038/ja.2006.7","title":"The Complete Biosynthetic Gene Cluster of the 28-Membered Polyketide Macrolactones, Halstoctacosanolides, from Streptomyces halstedii HC34","abstract":null,"journal":"The Journal of Antibiotics","year":2006,"id":673637,"datarank":0.47032413238937254,"base_score":3.1354942159291497,"endowment":3.1354942159291497,"self_citation_contribution":0.47032413238937254,"citation_network_contribution":0.0,"self_endowment_contribution":0.47032413238937254,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":22,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1759996,"name":"Katsumi Kakinuma","orcid":null,"position":1,"is_corresponding":false},{"id":1759997,"name":"Tadashi Eguchi","orcid":null,"position":2,"is_corresponding":false},{"id":1759995,"name":"Shigehiro Tohyama","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"The Complete Biosynthetic Gene Cluster of the 28-Membered Polyketide Macrolactones, Halstoctacosanolides, from Streptomyces halstedii HC34","abstract":"Halstoctacoanolides A and B are 28-membered polyketide macrolactones and were isolated from Streptomyces halstedii HC34. The biosynthetic gene cluster (hls cluster) of halstoctacosanolides was completely identified from the genome library of Streptomyces halstedii HC34. DNA sequence analysis of ca. 100 kb region revealed that there were seven type I polyketide synthases (PKSs) and two cytochrome P450 monooxygenases in this cluster. Involvement of the gene cluster in the halstoctacosanolide biosynthesis was demonstrated by the gene disruption of P450 monooxygenase genes. The mutants produced a new deoxygenated halstoctacosanolide derivative, halstoctacosanolide C, which confirmed that the hls gene cluster was essential for the biosynthesis of halstoctacosanolides.","is_dataset_classified":null,"base_score":3.1354942159291497,"endowment":3.1354942159291497,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"16568718","pmcid":null,"openalex_id":"https://openalex.org/W2038589675","authors":[],"funders":[],"total_grants":0,"fwci":0.4674,"citation_percentile":0.70169522,"influential_citations":0,"citation_trend":[{"year":2012,"count":2},{"year":2013,"count":2},{"year":2014,"count":2},{"year":2017,"count":3},{"year":2018,"count":3},{"year":2021,"count":1},{"year":2022,"count":1},{"year":2023,"count":2}],"oa_status":"bronze","license":"http://www.springer.com/tdm","oa_locations":[{"url":"https://www.nature.com/articles/ja20067.pdf","host_type":"journal"},{"url":"https://www.nature.com/articles/ja20067.pdf","host_type":"publisher"},{"url":"http://www.nature.com/articles/ja20067.pdf","host_type":"publisher"},{"url":"http://www.nature.com/articles/ja20067","host_type":"publisher"},{"url":"https://doi.org/10.1038/ja.2006.7","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/16568718","host_type":"repository"},{"url":"https://pubmed.ncbi.nlm.nih.gov/16673583","host_type":"repository"}],"fields_of_study":["Microbial Natural Products and Biosynthesis","Fungal Biology and Applications","Plant biochemistry and biosynthesis","Chemical Phenomena","Chemistry, Physical","Chromatography, Thin Layer","Cloning, Molecular","DNA, Fungal","Databases, Genetic","In Situ Hybridization","Macrolides","Magnetic Resonance Spectroscopy","Mixed Function Oxygenases","Multigene Family","Optical Rotation","Plasmids","Spectrophotometry, Infrared","Spectrophotometry, Ultraviolet","Streptomyces"],"mesh_terms":["Chemistry, Physical","Chromatography, Thin Layer","Cloning, Molecular","DNA, Fungal","Multigene Family","Mixed Function Oxygenases","Magnetic Resonance Spectroscopy","Optical Rotation","Plasmids","Spectrophotometry, Infrared","Spectrophotometry, Ultraviolet","Streptomyces","In Situ Hybridization","Macrolides","Databases, Genetic","Chemical Phenomena","Animals","Oxidoreductases Acting on CH-CH Group Donors","Anthelmintics","Insecticides","Chitinases"],"keywords":["Gene cluster","Polyketide","Biology","Streptomyces","Monooxygenase","Polyketide synthase","Gene","Genetics","Biosynthesis","Cytochrome P450","Biochemistry","Enzyme"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-16T14:04:54.386240Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}