{"doi":"10.1038/gt.2010.81","title":"Surface immobilization of hexa-histidine-tagged adeno-associated viral vectors for localized gene delivery","abstract":null,"journal":"Gene Therapy","year":2010,"id":596471,"datarank":0.47032413238937254,"base_score":3.1354942159291497,"endowment":3.1354942159291497,"self_citation_contribution":0.47032413238937254,"citation_network_contribution":0.0,"self_endowment_contribution":0.47032413238937254,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":22,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1527638,"name":"J T Koerber","orcid":null,"position":1,"is_corresponding":false},{"id":1527640,"name":"K Gujraty","orcid":null,"position":2,"is_corresponding":false},{"id":1527643,"name":"S R Bethi","orcid":null,"position":3,"is_corresponding":false},{"id":1527644,"name":"R S Kane","orcid":null,"position":4,"is_corresponding":false},{"id":1527645,"name":"D V Schaffer","orcid":null,"position":5,"is_corresponding":false},{"id":1527636,"name":"J-H Jang","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Surface immobilization of hexa-histidine-tagged adeno-associated viral vectors for localized gene delivery","abstract":"Adeno-associated viral (AAV) vectors, which are undergoing broad exploration in clinical trials, have significant promise for therapeutic gene delivery because of their safety and delivery efficiency. Gene delivery technologies capable of mediating localized gene expression may further enhance the potential of AAV in a variety of therapeutic applications by reducing spread outside a target region, which may thereby reduce off-target side effects. We have genetically engineered an AAV variant capable of binding to surfaces with high affinity through a hexa-histidine metal-binding interaction. This immobilized AAV vector system mediates high-efficiency delivery to cells that contact the surface and thus may have promise for localized gene delivery, which may aid numerous applications of AAV delivery to gene therapy.","is_dataset_classified":null,"base_score":3.1354942159291497,"endowment":3.1354942159291497,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"20508598","pmcid":"PMC2932747","openalex_id":"https://openalex.org/W2144336433","authors":[],"funders":[{"funder_name":"NHLBI NIH HHS","grant_id":"R01HL081527","title":null},{"funder_name":"National Institutes of Health","grant_id":"5R01HL081527-04","title":"Engineering AAV Vectors to Evade Antibody Neutralization"}],"total_grants":2,"fwci":1.9307,"citation_percentile":0.87096171,"influential_citations":0,"citation_trend":[{"year":2012,"count":2},{"year":2013,"count":5},{"year":2014,"count":3},{"year":2015,"count":1},{"year":2016,"count":1},{"year":2017,"count":1},{"year":2019,"count":2},{"year":2020,"count":1},{"year":2023,"count":1},{"year":2026,"count":1}],"oa_status":"green","license":"Springer TDM","oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2932747","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2932747","host_type":"repository"},{"url":"https://www.nature.com/articles/gt201081.pdf","host_type":"publisher"},{"url":"https://www.nature.com/articles/gt201081","host_type":"publisher"},{"url":"https://doi.org/10.1038/gt.2010.81","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/20508598","host_type":"repository"},{"url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.774.7480","host_type":""},{"url":"https://escholarship.org/uc/item/0jn8327b","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC2932747","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC2932747?pdf=render","host_type":"Europe_PMC"},{"url":"http://dx.doi.org/10.1038/gt.2010.81","host_type":""},{"url":"https://dx.doi.org/10.1038/gt.2010.81","host_type":""},{"url":"https://doi.org/https://doi.org/10.1038/gt.2010.81","host_type":""}],"fields_of_study":["Virus-based gene therapy research","RNA Interference and Gene Delivery","Viral Infectious Diseases and Gene Expression in Insects","0301 basic medicine","03 medical and health sciences","0303 health sciences"],"mesh_terms":["Dependovirus","Cells, Cultured","Genetic Vectors","Histidine","Humans","Oligopeptides","Transduction, Genetic","Genetic Therapy","Gene Transfer Techniques"],"keywords":["Gene delivery","Genetic enhancement","Adeno-associated virus","Vector (molecular biology)","Viral vector","Delivery system","Biology","Gene","Transfection","Computational biology","Virology","Genetics","Pharmacology","Recombinant DNA","Cells","Genetic Vectors","localized gene delivery","Medical and Health Sciences","Article","Transduction","Genetic","hexa-histidine","Transduction, Genetic","Humans","Histidine","Cells, Cultured","Cultured","5.2 Cellular and gene therapies","Gene Transfer Techniques","AAV","Gene Therapy","Genetic Therapy","Biological Sciences","Dependovirus","substrate-mediated gene delivery","Development of treatments and therapeutic interventions","Oligopeptides","Biotechnology"],"sdg_mappings":[{"sdg_number":3,"sdg_label":"3. 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