{"doi":"10.1038/cddis.2010.69","title":"The CD14+/lowCD16+ monocyte subset is more susceptible to spontaneous and oxidant-induced apoptosis than the CD14+CD16− subset","abstract":"Human monocytes can be classified into two subsets with distinctive characteristics. In this study, we report a difference in apoptotic potential between these two subsets with CD14(+/low)CD16(+) monocytes being more susceptible than CD14(+)CD16(-) monocytes to undergo spontaneous apoptosis and apoptosis induced by reactive oxygen species (ROS). By global transcriptomic and proteomic approaches, we observed that CD14(+/low)CD16(+) monocytes expressed higher levels of pro-apoptotic genes and proteins such as TNFα, caspase 3, Bax and cytochrome c and showed more caspases 3 and 7 activities. They also exhibited greater aerobic respiration resulting in a higher production of ROS from the mitochondria. CD14(+)CD16(-) monocytes, in contrast, showed higher expression of glutathione (GSH)-metabolizing genes such as GSH peroxidase and microsomal GSH S-transferase and were more resistant to oxidative stress than CD14(+/low)CD16(+) monocytes. The apoptosis of CD14(+/low)CD16(+) monocytes was ROS dependent as reducing ROS levels significantly reduced cell death. This is the first report of a differential apoptotic propensity of human monocyte subsets, and gaining a better understanding of this process may help to provide a better understanding of the roles of these subsets during homeostasis and under pathological conditions, particularly in situations in which high levels of oxidants are present.","journal":"Cell Death & Disease","year":2010,"id":4161,"datarank":0.6010999777848708,"base_score":4.007333185232471,"endowment":4.007333185232471,"self_citation_contribution":0.6010999777848708,"citation_network_contribution":0.0,"self_endowment_contribution":0.6010999777848708,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":54,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.0367,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2010-11-04","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":41898,"name":"Y.-C. Tan","orcid":null,"position":1,"is_corresponding":false},{"id":41899,"name":"W-C Wong","orcid":null,"position":2,"is_corresponding":false},{"id":41900,"name":"X Sem","orcid":null,"position":3,"is_corresponding":false},{"id":10782,"name":"H. Zhang","orcid":null,"position":4,"is_corresponding":false},{"id":41263,"name":"H. Han","orcid":"0000-0003-2881-4420","position":5,"is_corresponding":false},{"id":41901,"name":"S-M Ong","orcid":null,"position":6,"is_corresponding":false},{"id":41902,"name":"K-L Wong","orcid":null,"position":7,"is_corresponding":false},{"id":41903,"name":"W-H Yeap","orcid":null,"position":8,"is_corresponding":false},{"id":41904,"name":"S-K Sze","orcid":null,"position":9,"is_corresponding":false},{"id":41905,"name":"P Kourilsky","orcid":null,"position":10,"is_corresponding":false},{"id":41906,"name":"S-C Wong","orcid":null,"position":11,"is_corresponding":false},{"id":41907,"name":"Chen Zhao","orcid":"0000-0001-7270-7580","position":12,"is_corresponding":false},{"id":41908,"name":"W. Wei‐Lynn Wong","orcid":"0000-0003-3155-1211","position":13,"is_corresponding":false},{"id":41909,"name":"Xiaohui Sem","orcid":"0000-0002-4962-9825","position":14,"is_corresponding":false},{"id":41910,"name":"Huoming Zhang","orcid":"0000-0001-5416-0358","position":15,"is_corresponding":false},{"id":41911,"name":"Han Han","orcid":"0000-0003-2222-5270","position":16,"is_corresponding":false},{"id":41912,"name":"Siew‐Min Ong","orcid":"0000-0001-7929-984X","position":17,"is_corresponding":false},{"id":41913,"name":"KL Wong","orcid":null,"position":18,"is_corresponding":false},{"id":41914,"name":"Siu Kwan Sze","orcid":"0000-0002-5652-1687","position":19,"is_corresponding":false},{"id":41915,"name":"Philippe Kourilsky","orcid":null,"position":20,"is_corresponding":false},{"id":41897,"name":"C Zhao","orcid":"0000-0001-5825-2401","position":0,"is_corresponding":true}],"reference_count":40,"raw_metadata":null,"created_at":"2026-03-01T18:20:47.508186Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}