{"doi":"10.1038/25786","title":"RNA polymerase II is an essential mRNA polyadenylation factor","abstract":null,"journal":"Nature","year":1998,"id":633686,"datarank":0.882498358273242,"base_score":5.883322388488279,"endowment":5.883322388488279,"self_citation_contribution":0.882498358273242,"citation_network_contribution":0.0,"self_endowment_contribution":0.882498358273242,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":358,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":5592,"name":"James L. Manley","orcid":"0000-0002-8341-1459","position":1,"is_corresponding":false},{"id":1508279,"name":"Yutaka Hirose","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"RNA polymerase II is an essential mRNA polyadenylation factor","abstract":"Production of messenger RNA in eukaryotic cells is a complex, multistep process. mRNA polyadenylation, or 3' processing, requires several protein factors, including cleavage/polyadenylation-specificity factor (CPSF), cleavage-stimulation factor, two cleavage factors and poly(A) polymerase. These proteins seem to be unnecessary for other steps in mRNA synthesis such as transcription and splicing, and factors required for these processes were not considered to be essential for polyadenylation. Nonetheless, these reactions may be linked so that they are effectively coordinated in vivo. For example, the CTD carboxy-terminal domain of the largest subunit of RNA polymerase II (RNAP II) is required for efficient splicing and polyadenylation in vivo, and CPSF is brought to a promoter by the transcription factor TFIID and transferred to RNAP II at the time of transcription initiation. These findings suggest that polyadenylation factors can be recruited to an RNA 3'-processing signal by RNAP II, where they dissociate from the polymerase and initiate polyadenylation. Here we present results that extend this model by showing that RNAP II is actually required, in the absence of transcription, for 3' processing in vitro.","is_dataset_classified":null,"base_score":5.883322388488279,"endowment":5.883322388488279,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"9738505","pmcid":null,"openalex_id":"https://openalex.org/W1673271895","authors":[],"funders":[],"total_grants":0,"fwci":10.7047,"citation_percentile":0.98933806,"influential_citations":0,"citation_trend":[{"year":2012,"count":12},{"year":2013,"count":9},{"year":2014,"count":16},{"year":2015,"count":5},{"year":2016,"count":9},{"year":2017,"count":7},{"year":2018,"count":7},{"year":2019,"count":7},{"year":2020,"count":2},{"year":2021,"count":5},{"year":2022,"count":8},{"year":2023,"count":4},{"year":2024,"count":3},{"year":2025,"count":2}],"oa_status":"closed","license":"http://www.springer.com/tdm","oa_locations":[{"url":"http://www.nature.com/articles/25786.pdf","host_type":"publisher"},{"url":"http://www.nature.com/articles/25786","host_type":"publisher"},{"url":"https://doi.org/10.1038/25786","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/9738505","host_type":"repository"},{"url":"https://www.nature.com/articles/25786","host_type":"repository"}],"fields_of_study":["RNA Research and Splicing","RNA and protein synthesis mechanisms","RNA modifications and cancer","Animals","Binding Sites","Cattle","Escherichia coli","HeLa Cells","Humans","Macromolecular Substances","Phosphoproteins","Phosphorylation","Poly A","Protein Processing, Post-Translational","RNA Polymerase II","RNA, Messenger","Recombinant Proteins","Transcription, Genetic"],"mesh_terms":["Animals","Binding Sites","Cattle","Escherichia coli","HeLa Cells","Humans","Phosphoproteins","Phosphorylation","Poly A","Protein Processing, Post-Translational","Recombinant Proteins","RNA Polymerase II","RNA, Messenger","Transcription, Genetic","Macromolecular Substances","Hela Cells"],"keywords":["Cleavage and polyadenylation specificity factor","Polyadenylation","Post-transcriptional modification","Cleavage factor","RNA polymerase II","Cleavage stimulation factor","Transcription factor II D","RNA splicing","Terminator (solar)","Cell biology","Biology","Precursor mRNA","Transcription (linguistics)","Transcription factor II F","Antitermination","RNA polymerase II holoenzyme","Messenger RNA","RNA","RNA polymerase","Genetics","Promoter","Gene expression","Gene","Physics"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-06T12:29:21.084010Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}