{"doi":"10.1034/j.1600-0854.2002.30705.x","title":"GBF1, a Guanine Nucleotide Exchange Factor for ADP‐Ribosylation Factors, is Localized to the <i>cis</i>‐Golgi and Involved in Membrane Association of the COPI Coat","abstract":"<jats:p>Formation of coated carrier vesicles, such as COPI‐coated vesicles from the <jats:italic>cis</jats:italic>‐Golgi, is triggered by membrane binding of the GTP‐bound form of ADP‐ribosylation factors. This process is blocked by brefeldin A, which is an inhibitor of guanine nucleotide exchange factors for ADP‐ribosylation factor. GBF1 is one of the guanine nucleotide‐exchange factors for ADP‐ribosylation factor and is localized in the Golgi region. In the present study, we have determined the detailed subcellular localization of GBF1. Immunofluorescence microscopy of cells treated with nocodazole or incubated at 15 °C has suggested that GBF1 behaves similarly to proteins recycling between the <jats:italic>cis</jats:italic>‐Golgi and the endoplasmic reticulum. Immunoelectron microscopy has revealed that GBF1 localizes primarily to vesicular and tubular structures apposed to the <jats:italic>cis</jats:italic>‐face of Golgi stacks and minor fractions to the Golgi stacks. GBF1 overexpressed in cells causes recruitment of class I and class II ADP‐ribosylation factors onto Golgi membranes. Furthermore, overexpressed GBF1 antagonizes various effects of brefeldin A, such as inhibition of membrane recruitment of ADP‐ribosylation factors and the COPI coat, and redistribution of Golgi‐resident and itinerant proteins. These observations indicate that GBF1 is involved in the formation of COPI‐coated vesicles from the <jats:italic>cis</jats:italic>‐Golgi or the pre‐Golgi intermediate compartment through activating ADP‐ribosylation factors.</jats:p>","journal":"Traffic","year":2002,"id":30944,"datarank":6.475670323680162,"base_score":4.948759890378168,"endowment":4.948759890378168,"self_citation_contribution":0.7423139835567254,"citation_network_contribution":5.733356340123437,"self_endowment_contribution":0.7423139835567254,"citer_contribution":5.733356340123437,"corpus_percentile":null,"corpus_rank":null,"citation_count":140,"citer_count":135,"citers_with_citation_signal":120,"citers_with_endowment":120,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":166978,"name":"Yusaku Yoshida","orcid":null,"position":1,"is_corresponding":false},{"id":166979,"name":"Hideaki Tamaki","orcid":null,"position":2,"is_corresponding":false},{"id":166980,"name":"Seiji Torii","orcid":null,"position":3,"is_corresponding":false},{"id":166981,"name":"Chisa Shinotsuka","orcid":null,"position":4,"is_corresponding":false},{"id":166983,"name":"Shohei Yamashina","orcid":null,"position":5,"is_corresponding":false},{"id":166985,"name":"Kazuhisa Nakayama","orcid":null,"position":6,"is_corresponding":false},{"id":166977,"name":"Kazumasa Kawamoto","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":4.948759890378168,"endowment":4.948759890378168,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"12047556","pmcid":null,"openalex_id":"https://openalex.org/W2029581399","authors":[],"funders":[],"total_grants":0,"fwci":2.1378,"citation_percentile":0.85591625,"influential_citations":22,"citation_trend":[{"year":2012,"count":7},{"year":2013,"count":13},{"year":2014,"count":3},{"year":2015,"count":4},{"year":2016,"count":2},{"year":2017,"count":5},{"year":2018,"count":6},{"year":2019,"count":6},{"year":2020,"count":6},{"year":2021,"count":6},{"year":2022,"count":6},{"year":2023,"count":3},{"year":2024,"count":3},{"year":2025,"count":3},{"year":2026,"count":3}],"oa_status":"bronze","license":"http://onlinelibrary.wiley.com/termsAndConditions#vor","oa_locations":[{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1034/j.1600-0854.2002.30705.x","host_type":"journal"},{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1034/j.1600-0854.2002.30705.x","host_type":"BRONZE"},{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1034/j.1600-0854.2002.30705.x","host_type":"publisher"},{"url":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1034%2Fj.1600-0854.2002.30705.x","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/pdf/10.1034/j.1600-0854.2002.30705.x","host_type":"publisher"},{"url":"https://doi.org/10.1034/j.1600-0854.2002.30705.x","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/12047556","host_type":"repository"}],"fields_of_study":["Cellular transport and secretion","Lipid Membrane Structure and Behavior","Calcium signaling and nucleotide metabolism","Biology","Medicine","ADP-Ribosylation Factors","Animals","Antibody Specificity","Brefeldin A","COP-Coated Vesicles","Cell Line","Coat Protein Complex I","Golgi Apparatus","Guanine Nucleotide Exchange Factors","HeLa Cells","Humans","Microscopy, Immunoelectron","Rats","Recombinant Fusion Proteins","Subcellular Fractions"],"mesh_terms":["Animals","Antibody Specificity","Cell Line","Golgi Apparatus","HeLa Cells","Humans","Recombinant Fusion Proteins","Subcellular Fractions","Microscopy, Immunoelectron","Brefeldin A","Guanine Nucleotide Exchange Factors","ADP-Ribosylation Factors","Coat Protein Complex I","COP-Coated Vesicles","Rats","Hela Cells"],"keywords":["ADP ribosylation factor","Golgi apparatus","COPI","Brefeldin A","Endoplasmic reticulum","Cell biology","Biology","Golgi membrane","Guanine nucleotide exchange factor","Vesicle","COPII","Biochemistry","GTPase","Secretory pathway","Membrane"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-09T05:59:03.145615Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}