{"doi":"10.1021/jf049325f","title":"Role of Amyloid Type Cross β-Structure in the Formation of Soluble Aggregate and Gel in Heat-Induced Ovalbumin","abstract":null,"journal":"Journal of Agricultural and Food Chemistry","year":2005,"id":618837,"datarank":0.5926865577872142,"base_score":3.9512437185814275,"endowment":3.9512437185814275,"self_citation_contribution":0.5926865577872142,"citation_network_contribution":0.0,"self_endowment_contribution":0.5926865577872142,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":51,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1596723,"name":"A. Mukai","orcid":null,"position":1,"is_corresponding":false},{"id":1596724,"name":"A. Kato","orcid":null,"position":2,"is_corresponding":false},{"id":1596722,"name":"H. Azakami","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Role of Amyloid Type Cross β-Structure in the Formation of Soluble Aggregate and Gel in Heat-Induced Ovalbumin","abstract":"The heat-induced denaturation curve of ovalbumin followed by the ellipticity at 222 nm in circular dichroism spectra was consistent with that monitored by fluorescence with thioflavin T, which is an indication of amyloid fibril formation, while other proteins such as lysozyme and ovotransferrin did not fluoresce with thioflavin T during heat denaturation. The amount of soluble aggregate formed during heat denaturation was proportional to the increase in fluorescence with thioflavin T. The binding of soluble aggregates with thioflavin T was greatly suppressed in heat-denatured ovalbumin in the presence of thioflavin T. The similar inhibition effect of thioflavin T on the gel formation of heat-induced ovalbumin was observed. These results suggest that the amyloidogenic intermolecular beta-structure is involved in the formation of soluble aggregate and gel of heat-induced ovalbumin.","is_dataset_classified":null,"base_score":3.9512437185814275,"endowment":3.9512437185814275,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"15713049","pmcid":null,"openalex_id":"https://openalex.org/W1992067192","authors":[],"funders":[],"total_grants":0,"fwci":0.4918,"citation_percentile":0.63923411,"influential_citations":0,"citation_trend":[{"year":2012,"count":5},{"year":2013,"count":3},{"year":2014,"count":1},{"year":2015,"count":3},{"year":2016,"count":3},{"year":2017,"count":1},{"year":2018,"count":5},{"year":2019,"count":2},{"year":2020,"count":4},{"year":2021,"count":1},{"year":2022,"count":3},{"year":2023,"count":2},{"year":2024,"count":2},{"year":2025,"count":1},{"year":2026,"count":1}],"oa_status":"closed","license":null,"oa_locations":[{"url":"https://pubs.acs.org/doi/pdf/10.1021/jf049325f","host_type":"publisher"},{"url":"https://doi.org/10.1021/jf049325f","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/15713049","host_type":"repository"}],"fields_of_study":["Biochemical effects in animals","Proteins in Food Systems","Advanced Glycation End Products research"],"mesh_terms":["Amyloid","Fluorescent Dyes","Gels","Hot Temperature","Hydrogen-Ion Concentration","Ovalbumin","Protein Denaturation","Spectrometry, Fluorescence","Thiazoles","Protein Structure, Secondary","Benzothiazoles"],"keywords":["Thioflavin","Ovalbumin","Chemistry","Lysozyme","Denaturation (fissile materials)","Circular dichroism","Ovotransferrin","Protein aggregation","Fibril","Amyloid (mycology)","Biophysics","Biochemistry","Nuclear chemistry","Inorganic chemistry"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Clean water and sanitation"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-03T05:23:40.065965Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}