{"doi":"10.1021/jacsau.4c00307","title":"Chemoenzymatic Synthesis of Sulfated <i>N</i>-Glycans Recognized by Siglecs and Other Glycan-Binding Proteins","abstract":"High Resolution Image Download MS PowerPoint Slide Sulfated N -glycans are present in many glycoproteins, which are implicated in playing important roles in biological recognition processes. Here, we report the systematic chemoenzymatic synthesis of a library of sulfated and sialylated biantennary N -glycans and assess their binding to Siglecs and glycan-specific antibodies that recognize them as glycan ligands. The combined use of three human sulfotransferases, GlcNAc-6- O -sulfotransferase (CHST2), Gal-3- O -sulfotransferase (Gal3ST1), and keratan sulfate Gal-6- O -sulfotransferase (CHST1), resulted in asymmetric and symmetric branch-selective sulfation of the GlcNAc and/or Gal moieties of N -glycans. The extension of the sugar chain using α-2,3- and α-2,6-sialyltransferases afforded the sulfated and sialylated N -glycans. These synthetic glycans with different patterns of sulfation and sialylation were evaluated for binding to selected Siglecs and sulfoglycan-specific antibodies using glycan microarrays. The results confirm previously documented glycan-recognizing properties and further reveal novel specificities for these glycan-binding proteins, demonstrating the utility of the library for assessing the specificity of glycan-binding proteins recognizing sulfated and sialylated glycans.","journal":"JACS Au","year":2024,"id":427443,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":15,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9464,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1228128,"name":"Eleanor E. Bashian","orcid":null,"position":1,"is_corresponding":false},{"id":355882,"name":"Guanghui Zong","orcid":"0000-0002-7335-039X","position":2,"is_corresponding":false},{"id":292835,"name":"Corwin M. Nycholat","orcid":"0000-0001-5943-5357","position":3,"is_corresponding":false},{"id":313998,"name":"Ryan McBride","orcid":"0000-0001-8616-1910","position":4,"is_corresponding":false},{"id":1071143,"name":"Margaryta Gomozkova","orcid":null,"position":5,"is_corresponding":false},{"id":1084478,"name":"Shengyang Wang","orcid":"0000-0002-8454-1255","position":6,"is_corresponding":false},{"id":670735,"name":"Chin Huang","orcid":"0000-0002-4296-0400","position":7,"is_corresponding":false},{"id":296105,"name":"Digantkumar Chapla","orcid":"0000-0003-0649-1368","position":8,"is_corresponding":false},{"id":1227611,"name":"Edward N. Schmidt","orcid":"0000-0003-0729-5473","position":9,"is_corresponding":false},{"id":296111,"name":"Matthew S. Macauley","orcid":"0000-0003-4579-1048","position":10,"is_corresponding":false},{"id":53135,"name":"Kelley W. Moremen","orcid":"0000-0003-1768-582X","position":11,"is_corresponding":false},{"id":230746,"name":"James C. Paulson","orcid":"0000-0003-4589-5322","position":12,"is_corresponding":false},{"id":355883,"name":"Lai‐Xi Wang","orcid":"0000-0003-4293-5819","position":13,"is_corresponding":false},{"id":255903,"name":"Kun Huang","orcid":"0000-0002-9100-9638","position":0,"is_corresponding":true}],"reference_count":55,"raw_metadata":null,"created_at":"2026-07-19T01:58:47.805440Z","pmid":"39211606","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}