{"doi":"10.1021/jacs.7b00070","title":"Enzyme-Instructed Assembly and Disassembly Processes for Targeting Downregulation in Cancer Cells","abstract":null,"journal":"Journal of the American Chemical Society","year":2017,"id":596165,"datarank":0.7525919755222388,"base_score":5.017279836814924,"endowment":5.017279836814924,"self_citation_contribution":0.7525919755222388,"citation_network_contribution":0.0,"self_endowment_contribution":0.7525919755222388,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":150,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":452951,"name":"Huaimin Wang","orcid":"0000-0002-8796-0367","position":1,"is_corresponding":false},{"id":1357647,"name":"Rong Zhou","orcid":"0009-0009-3038-1514","position":2,"is_corresponding":false},{"id":642886,"name":"Jie Li","orcid":"0000-0002-5271-8263","position":3,"is_corresponding":false},{"id":291113,"name":"Bing Xu","orcid":"0000-0002-4639-387X","position":4,"is_corresponding":false},{"id":452952,"name":"Zhaoqianqi Feng","orcid":"0000-0002-1262-385X","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Enzyme-Instructed Assembly and Disassembly Processes for Targeting Downregulation in Cancer Cells","abstract":"Cancer cells differ from normal cells in both gain of functions (i.e., upregulation) and loss of functions (i.e., downregulation). While it is common to suppress gain of function for chemotherapy, it remains challenging to target downregulation in cancer cells. Here we show the combination of enzyme-instructed assembly and disassembly to target downregulation in cancer cells by designing peptidic precursors as the substrates of both carboxylesterases (CESs) and alkaline phosphatases (ALPs). The precursors turn into self-assembling molecules to form nanofibrils upon dephosphorylation by ALP, but CES-catalyzed cleavage of the ester bond on the molecules results in disassembly of the nanofibrils. The precursors selectively inhibit the cancer cells that downregulate CES (e.g., OVSAHO) but are innocuous to a hepatocyte that overexpresses CES (HepG2), while the two cell lines exhibit comparable ALP activities. This work illustrates a potential approach for the development of chemotherapy via targeting downregulation (or loss of functions) in cancer cells.","is_dataset_classified":null,"base_score":5.017279836814924,"endowment":5.017279836814924,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"28257192","pmcid":"PMC5380121","openalex_id":"https://openalex.org/W2592007767","authors":[],"funders":[{"funder_name":"National Cancer Institute","grant_id":"R01CA142746","title":null},{"funder_name":"Division of Materials Research","grant_id":"MRSEC-1420382","title":null},{"funder_name":"National Science Foundation","grant_id":"1420382","title":"Bioinspired Soft Materials"},{"funder_name":"National Institutes of Health","grant_id":"5R01CA142746-12","title":"Subcellular enzyme-instructed self-assembly for molecular anticancer nanomedicines"},{"funder_name":"National Institutes of Health","grant_id":"5R01CA142746-10","title":"Enzyme-instructed self-assembly for molecular anticancer nanomedicines"}],"total_grants":5,"fwci":7.7563,"citation_percentile":0.98514782,"influential_citations":0,"citation_trend":[{"year":2017,"count":16},{"year":2018,"count":24},{"year":2019,"count":13},{"year":2020,"count":19},{"year":2021,"count":21},{"year":2022,"count":16},{"year":2023,"count":11},{"year":2024,"count":13},{"year":2025,"count":12},{"year":2026,"count":5}],"oa_status":"hybrid","license":"cc-by","oa_locations":[{"url":"https://doi.org/10.1021/jacs.7b00070","host_type":"journal"},{"url":"https://doi.org/10.1021/jacs.7b00070","host_type":"publisher"},{"url":"http://pubs.acs.org/doi/pdf/10.1021/jacs.7b00070","host_type":"publisher"},{"url":"https://pubs.acs.org/doi/pdf/10.1021/jacs.7b00070","host_type":"publisher"},{"url":"https://pubmed.ncbi.nlm.nih.gov/28257192","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/5380121","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC5380121","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC5380121?pdf=render","host_type":"Europe_PMC"},{"url":"http://dx.doi.org/10.1021/jacs.7b00070","host_type":""},{"url":"https://dx.doi.org/10.1021/jacs.7b00070","host_type":""}],"fields_of_study":["Supramolecular Self-Assembly in Materials","Lipid Membrane Structure and Behavior","RNA Interference and Gene Delivery","02 engineering and technology","01 natural sciences","0104 chemical sciences","0210 nano-technology"],"mesh_terms":["Alkaline Phosphatase","Carboxylic Ester Hydrolases","Female","Humans","Liver Neoplasms","Ovarian Neoplasms","Down-Regulation","Cell Line, Tumor","Hep G2 Cells"],"keywords":["Downregulation and upregulation","Chemistry","Cancer cell","Dephosphorylation","Cell biology","Enzyme","Cancer","Phosphatase","Biochemistry","Biology","Ovarian Neoplasms","Liver Neoplasms","Down-Regulation","Hep G2 Cells","Alkaline Phosphatase","Cell Line, Tumor","Humans","Female","Carboxylic Ester Hydrolases"],"sdg_mappings":[{"sdg_number":3,"sdg_label":"3. Good health"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-28T03:55:30.580609Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}