{"doi":"10.1021/jacs.4c17659","title":"Quantitative Proteomics Identifies Profilin-1 as a Pseudouridine-Binding Protein","abstract":"High Resolution Image Download MS PowerPoint Slide Pseudouridine (Ψ) is the most abundant RNA modification in nature; however, not much is known about the biological functions of this modified nucleoside. Employing an unbiased quantitative proteomics method, we identified multiple candidate reader proteins of Ψ in RNA, including a cytoskeletal protein profilin-1 (PFN1). We demonstrated that PFN1 binds directly and selectively to Ψ-containing RNA. Additionally, we discovered approximately 4000 binding sites of PFN1 in human cells, including a known dyskerin (DKC1)-installed Ψ site in TPI1 mRNA, which encodes triosephosphate isomerase. Moreover, we showed that PFN1 and DKC1 are crucial for regulating the stability and translation efficiency of TPI1 mRNA through modulating PFN1-Ψ interaction. Together, we identified PFN1 as a reader protein of Ψ in RNA and illustrated a potential role of PFN1-Ψ interaction in post-transcriptional regulation. These findings provide new insights into the functions of Ψ in RNA biology and in modulating the expression of an important metabolic enzyme.","journal":"Journal of the American Chemical Society","year":2024,"id":434546,"datarank":0.3958585994422889,"base_score":2.639057329615259,"endowment":2.639057329615259,"self_citation_contribution":0.3958585994422889,"citation_network_contribution":0.0,"self_endowment_contribution":0.3958585994422889,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":13,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9599,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":269777,"name":"Xiaoxia Dai","orcid":"0000-0002-3514-5061","position":1,"is_corresponding":false},{"id":269778,"name":"Jun Yuan","orcid":"0000-0002-8265-0239","position":2,"is_corresponding":false},{"id":846632,"name":"Shiyang He","orcid":"0000-0002-1849-2635","position":3,"is_corresponding":false},{"id":1177571,"name":"Kriti Shah","orcid":"0009-0007-4357-5049","position":4,"is_corresponding":false},{"id":1241344,"name":"Shiyuan Guo","orcid":"0000-0003-0726-9128","position":5,"is_corresponding":false},{"id":1241345,"name":"Zheng Duan","orcid":"0000-0002-0173-663X","position":6,"is_corresponding":false},{"id":571614,"name":"Jernej Murn","orcid":"0000-0002-1729-5859","position":7,"is_corresponding":false},{"id":110450,"name":"Yinsheng Wang","orcid":"0000-0001-5565-283X","position":8,"is_corresponding":false},{"id":1141351,"name":"Songbo Wei","orcid":"0000-0001-9311-1823","position":0,"is_corresponding":true}],"reference_count":37,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T01:59:58.582186Z","pmid":"39812085","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}