{"doi":"10.1021/jacs.2c10079","title":"Dissociative Transition State in Hepatitis Delta Virus Ribozyme Catalysis","abstract":"Ribonucleases and small nucleolytic ribozymes are both able to catalyze RNA strand cleavage through 2′- O -transphosphorylation, provoking the question of whether protein and RNA enzymes facilitate mechanisms that pass through the same or distinct transition states. Here, we report the primary and secondary 18 O kinetic isotope effects for hepatitis delta virus ribozyme catalysis that reveal a dissociative, metaphosphate-like transition state in stark contrast to the late, associative transition states observed for reactions catalyzed by specific base, Zn 2+ ions, or ribonuclease A. This new information provides evidence for a discrete ribozyme active site design that modulates the RNA cleavage pathway to pass through an altered transition state.","journal":"Journal of the American Chemical Society","year":2023,"id":345592,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":12,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.95,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":635960,"name":"Şölen Ekesan","orcid":"0000-0002-5598-5754","position":1,"is_corresponding":false},{"id":1085574,"name":"Hsuan-Chun Lin","orcid":"0000-0003-2220-0823","position":2,"is_corresponding":false},{"id":1066649,"name":"Shahbaz Gardezi","orcid":"0000-0001-7067-1924","position":3,"is_corresponding":false},{"id":345743,"name":"Nan‐Sheng Li","orcid":"0000-0002-1185-3688","position":4,"is_corresponding":false},{"id":345742,"name":"Timothy J. Giese","orcid":"0000-0002-0653-9168","position":5,"is_corresponding":false},{"id":553463,"name":"Erika McCarthy","orcid":"0000-0001-8089-0207","position":6,"is_corresponding":false},{"id":895981,"name":"Michael E. Harris","orcid":"0000-0001-8977-4392","position":7,"is_corresponding":false},{"id":61660,"name":"Darrin M. York","orcid":"0000-0002-9193-7055","position":8,"is_corresponding":false},{"id":345746,"name":"Joseph A. Piccirilli","orcid":"0000-0002-0541-6270","position":9,"is_corresponding":false},{"id":345741,"name":"Benjamin P. Weissman","orcid":"0000-0003-0804-0464","position":0,"is_corresponding":true}],"reference_count":61,"raw_metadata":null,"created_at":"2026-07-19T01:11:44.152490Z","pmid":"36706353","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}