{"doi":"10.1021/jacs.0c04328","title":"Investigation of Indigoidine Synthetase Reveals a Conserved Active-Site Base Residue of Nonribosomal Peptide Synthetase Oxidases","abstract":"Nonribosomal peptide synthetase (NRPS) oxidase (Ox) domains oxidize protein-bound intermediates to install crucial structural motifs in bioactive natural products. The mechanism of this domain remains elusive. Here, by studying indigoidine synthetase, a single-module NRPS involved in the biosynthesis of indigoidine and several other bacterial secondary metabolites, we demonstrate that its Ox domain utilizes an active-site base residue, tyrosine 665, to deprotonate a protein-bound l-glutaminyl residue. We further validate the generality of this active-site residue among NRPS Ox domains. These findings not only resolve the biosynthetic pathway mediated by indigoidine synthetase but enable mechanistic insight into NRPS Ox domains.","journal":"Journal of the American Chemical Society","year":2020,"id":63450,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":44,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9523,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":335304,"name":"Yan Chen","orcid":"0000-0002-1125-313X","position":1,"is_corresponding":false},{"id":335305,"name":"Fei Gan","orcid":"0000-0003-1670-5251","position":2,"is_corresponding":false},{"id":335990,"name":"Chunsheng Yan","orcid":null,"position":3,"is_corresponding":false},{"id":335306,"name":"Liyuan Jin","orcid":"0000-0001-6738-1424","position":4,"is_corresponding":false},{"id":335307,"name":"Jennifer Gin","orcid":"0000-0001-5636-7563","position":5,"is_corresponding":false},{"id":335308,"name":"Christopher J. Petzold","orcid":"0000-0002-8270-5228","position":6,"is_corresponding":false},{"id":271245,"name":"Jay D. Keasling","orcid":"0000-0003-4170-6088","position":7,"is_corresponding":false},{"id":335303,"name":"Bo Pang","orcid":"0000-0001-7110-6732","position":0,"is_corresponding":true}],"reference_count":32,"raw_metadata":null,"created_at":"2026-07-18T21:11:00.632961Z","pmid":"32510939","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}