{"doi":"10.1021/bi00202a023","title":"Initiation of Transcription at the Human Terminal Deoxynucleotidyl Transferase Gene Promoter: A Novel Role for the TATA Binding Protein","abstract":null,"journal":"Biochemistry","year":1994,"id":589359,"datarank":0.7343499247036699,"base_score":1.9459101490553132,"endowment":1.9459101490553132,"self_citation_contribution":0.29188652235829704,"citation_network_contribution":0.44246340234537285,"self_endowment_contribution":0.29188652235829704,"citer_contribution":0.44246340234537285,"corpus_percentile":null,"corpus_rank":null,"citation_count":6,"citer_count":6,"citers_with_citation_signal":6,"citers_with_endowment":6,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1507914,"name":"Baoli Yang","orcid":null,"position":1,"is_corresponding":false},{"id":229956,"name":"Dipa Bhaumik","orcid":null,"position":2,"is_corresponding":false},{"id":1507915,"name":"Theoni Trangas","orcid":null,"position":3,"is_corresponding":false},{"id":645786,"name":"Anne V. Philips","orcid":"0000-0003-2093-9413","position":4,"is_corresponding":false},{"id":1507916,"name":"Karen E. Chancellor","orcid":null,"position":5,"is_corresponding":false},{"id":1507917,"name":"Mary Sue Coleman","orcid":null,"position":6,"is_corresponding":false},{"id":608426,"name":"David H. Sorscher","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Initiation of Transcription at the Human Terminal Deoxynucleotidyl Transferase Gene Promoter: A Novel Role for the TATA Binding Protein","abstract":"Control of initiation of transcription of the human terminal deoxynucleotidyl transferase (TdT) gene was investigated by using an in vitro transcription assay. The precise contribution of discrete basal promoter elements to transcription initiation was determined by testing deletion and substitution mutations. The primary element, contained within the region spanning -34 to -14 bp relative to the transcription start site, accounted for 80% of basal promoter activity. TdT promoter activity required the sequence ACCCT at -24 to -20 bp since a dramatic decrease in transcription initiation was observed after mutation of this sequence, whereas mutation of the adjacent sequence from -32 to -25 bp did not alter promoter activity. The secondary element contained sequences surrounding the transcription start site and had 20% of promoter activity. Deletion of both elements completely abolished transcription initiation. Initiator characteristics of the secondary element were revealed by using the in vitro assay: promoter sequences at the transcription start site were sufficient to direct accurate initiation at a single site. Mutation of the sequence GGGTG spanning the transcription start site resulted in loss of transcription initiation. Both the primary and secondary elements were nonhomologous to corresponding regions from the mouse TdT gene promoter. While the human basal promoter functioned in the absence of TATA consensus sequences or GC-rich SP1 binding sites, it was dependent on active TFIID. In contrast to other TATA-less promoters, purified TATA binding protein substituted for the TFIID complex and restored promoter activity to TFIID-inactivated nuclear extracts.","is_dataset_classified":null,"base_score":1.9459101490553132,"endowment":1.9459101490553132,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"8086419","pmcid":null,"openalex_id":"https://openalex.org/W2040919061","authors":[],"funders":[{"funder_name":"NCI NIH HHS","grant_id":"R01CA19492","title":null}],"total_grants":1,"fwci":0.273,"citation_percentile":0.50739702,"influential_citations":0,"citation_trend":[],"oa_status":"closed","license":null,"oa_locations":[{"url":"https://pubs.acs.org/doi/pdf/10.1021/bi00202a023","host_type":"publisher"},{"url":"https://doi.org/10.1021/bi00202a023","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/8086419","host_type":"repository"},{"url":"https://ir.uiowa.edu/obgyn_pubs/1175","host_type":"repository"}],"fields_of_study":["CRISPR and Genetic Engineering","RNA and protein synthesis mechanisms","RNA Interference and Gene Delivery","Base Sequence","Cell Line","DNA Nucleotidylexotransferase","DNA Primers","DNA-Binding Proteins","HeLa Cells","Humans","Molecular Sequence Data","Mutagenesis","Mutation","Promoter Regions, Genetic","Sequence Deletion","TATA Box","TATA-Box Binding Protein","Transcription Factor TFIID","Transcription Factors","Transcription, Genetic"],"mesh_terms":["Base Sequence","Cell Line","DNA Nucleotidylexotransferase","DNA-Binding Proteins","HeLa Cells","Humans","Molecular Sequence Data","Mutation","Promoter Regions, Genetic","Transcription Factors","Transcription, Genetic","Mutagenesis","TATA Box","Sequence Deletion","DNA Primers","TATA-Box Binding Protein","Transcription Factor TFIID","Hela Cells"],"keywords":["Terminal deoxynucleotidyl transferase","TATA box","TATA-binding protein","Gene","Molecular biology","Transcription (linguistics)","Genetics","Biology","Promoter","Transcription factor","DNA-binding protein","Gene expression","TUNEL assay"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-23T17:06:43.049654Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}