{"doi":"10.1021/acsnano.1c02494","title":"Extraction of Viral Nucleic Acids with Carbon Nanotubes Increases SARS-CoV-2 Quantitative Reverse Transcription Polymerase Chain Reaction Detection Sensitivity","abstract":"The global SARS-CoV-2 coronavirus pandemic has led to a surging demand for rapid and efficient viral infection diagnostic tests, generating a supply shortage in diagnostic test consumables including nucleic acid extraction kits. Here, we develop a modular method for high-yield extraction of viral single-stranded nucleic acids by using “capture” ssDNA sequences attached to carbon nanotubes. Target SARS-CoV-2 viral RNA can be captured by ssDNA-nanotube constructs via hybridization and separated from the liquid phase in a single-tube system with minimal chemical reagents, for downstream quantitative reverse transcription polymerase chain reaction (RT-qPCR) detection. This nanotube-based extraction method enables 100% extraction yield of target SARS-CoV-2 RNA from phosphate-buffered saline in comparison to ∼20% extraction yield when using a commercial silica-column kit. Notably, carbon nanotubes enable extraction of nucleic acids directly from 50% human saliva with a similar efficiency as achieved with commercial DNA/RNA extraction kits, thereby bypassing the need for further biofluid purification and avoiding the use of commercial extraction kits. Carbon nanotube-based extraction of viral nucleic acids facilitates high-yield and high-sensitivity identification of viral nucleic acids such as the SARS-CoV-2 viral genome with a reduced reliance on reagents affected by supply chain obstacles.","journal":"ACS Nano","year":2021,"id":155771,"datarank":2.155903084600666,"base_score":4.060443010546419,"endowment":4.060443010546419,"self_citation_contribution":0.6090664515819629,"citation_network_contribution":1.546836633018703,"self_endowment_contribution":0.6090664515819629,"citer_contribution":1.546836633018703,"corpus_percentile":null,"corpus_rank":null,"citation_count":57,"citer_count":57,"citers_with_citation_signal":51,"citers_with_endowment":51,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9483,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":301842,"name":"Eduardo González‐Grandío","orcid":"0000-0002-2059-4542","position":1,"is_corresponding":false},{"id":555545,"name":"Nicole Navarro","orcid":"0000-0002-1353-2124","position":2,"is_corresponding":false},{"id":40709,"name":"Rebecca L. Pinals","orcid":"0000-0002-5369-2317","position":3,"is_corresponding":false},{"id":278347,"name":"Francis Ledesma","orcid":"0000-0002-6280-1394","position":4,"is_corresponding":false},{"id":260630,"name":"Darwin Yang","orcid":"0000-0002-6839-1955","position":5,"is_corresponding":false},{"id":260634,"name":"Markita P. Landry","orcid":"0000-0002-5832-8522","position":6,"is_corresponding":false},{"id":347063,"name":"Sanghwa Jeong","orcid":"0000-0003-3529-0346","position":0,"is_corresponding":true}],"reference_count":24,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T23:44:03.750995Z","pmid":"34105936","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}