{"doi":"10.1021/acschembio.0c00925","title":"Versatile Labeling and Detection of Endogenous Proteins Using Tag-Assisted Split Enzyme Complementation","abstract":"Recent advances in genome engineering have expanded our capabilities to study proteins in their natural states. In particular, the ease and scalability of knocking-in small peptide tags has enabled high throughput tagging and analysis of endogenous proteins. To improve enrichment capacities and expand the functionality of knock-ins using short tags, we developed the tag-assisted split enzyme complementation (TASEC) approach, which uses two orthogonal small peptide tags and their cognate binders to conditionally drive complementation of a split enzyme upon labeled protein expression. Using this approach, we have engineered and optimized the tag-assisted split HaloTag complementation system (TA-splitHalo) and demonstrated its versatile applications in improving the efficiency of knock-in cell enrichment, detection of protein-protein interaction, and isolation of biallelic gene edited cells through multiplexing.","journal":"ACS Chemical Biology","year":2021,"id":172383,"datarank":0.4887144807032224,"base_score":3.258096538021482,"endowment":3.258096538021482,"self_citation_contribution":0.4887144807032224,"citation_network_contribution":0.0,"self_endowment_contribution":0.4887144807032224,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":25,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9511,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":411732,"name":"David T. Brown","orcid":"0000-0001-7531-8230","position":1,"is_corresponding":false},{"id":267166,"name":"Rachel M. Rudlaff","orcid":"0000-0003-1191-9293","position":2,"is_corresponding":false},{"id":708511,"name":"Julia K. Doh","orcid":"0000-0001-9375-6947","position":3,"is_corresponding":false},{"id":585996,"name":"Struan Bourke","orcid":"0000-0003-1534-6736","position":4,"is_corresponding":false},{"id":323777,"name":"Yina Wang","orcid":"0009-0009-6568-0717","position":5,"is_corresponding":false},{"id":495632,"name":"Shuqin Zhou","orcid":"0000-0002-5027-9535","position":6,"is_corresponding":false},{"id":585997,"name":"Rasmi Cheloor-Kovilakam","orcid":"0000-0003-2793-0489","position":7,"is_corresponding":false},{"id":406504,"name":"Bo Huang","orcid":"0000-0003-1704-4141","position":8,"is_corresponding":false},{"id":585995,"name":"Suraj Makhija","orcid":"0000-0002-2490-2434","position":0,"is_corresponding":true}],"reference_count":45,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T23:46:41.424716Z","pmid":"33734687","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}