{"doi":"10.1021/acsami.5c12410","title":"Host-Gated Enzymatic Release (H-GER) Enables Colorimetric Transduction for Enzyme Measurement","abstract":"We introduce Host-Gated Enzymatic Release (H-GER) as an alternative colorimetric signal transduction mechanism for measuring amylase activity. This assay uses a visually colored complex formed when hydroxypropyl-γ-cyclodextrin (HP-γ-CD) binds to the aggregachromic dye CRANAD-2, with the HP side chains playing a key role in the complexation. The analytical capability of this visually addressable assay relies on changes in dye dispersity, triggered by the enzymatic release of gated CRANAD-2 from HP-γ-CD host. Upon cleavage of HP-γ-CD, the freed dye clusters in the aqueous environment, resulting in a sequence of color changes observed by the naked eye. The H-GER assay demonstrated a limit of detection of 154 U/mL for α-amylase. Analysis based on Michaelis–Menten kinetics and molecular dynamics simulations revealed that the H-GER assay exhibits good enzymatic specificity, despite showing reduced catalytic efficiency. These results demonstrate that H-GER is an effective and potentially valuable signal transduction mechanism that expands the current toolbox for developing in vitro colorimetric assays targeting specific enzymatic activities.","journal":"ACS Applied Materials & Interfaces","year":2025,"id":573903,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9533,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":225055,"name":"Wen Liu","orcid":"0000-0003-3434-4162","position":1,"is_corresponding":false},{"id":1481017,"name":"Qing Huang","orcid":"0000-0001-7083-9416","position":2,"is_corresponding":false},{"id":1481428,"name":"Zhong Xiang","orcid":null,"position":3,"is_corresponding":false},{"id":1481018,"name":"Jin Gu","orcid":"0000-0002-2489-4665","position":4,"is_corresponding":false},{"id":1481429,"name":"Ruby Segerman","orcid":null,"position":5,"is_corresponding":false},{"id":1481430,"name":"Jordan Choi","orcid":null,"position":6,"is_corresponding":false},{"id":1481019,"name":"X.W. Wang","orcid":"0000-0003-1272-675X","position":7,"is_corresponding":false},{"id":476574,"name":"Zhicheng Jin","orcid":"0000-0001-6072-7533","position":8,"is_corresponding":false},{"id":504704,"name":"Zeyu Zhang","orcid":"0000-0002-7796-2887","position":0,"is_corresponding":true}],"reference_count":69,"raw_metadata":null,"created_at":"2026-07-19T02:57:36.753600Z","pmid":"40891086","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}