{"doi":"10.1021/acsami.1c04751","title":"An In Situ Generated Prussian Blue Nanoparticle-Mediated Multimode Nanozyme-Linked Immunosorbent Assay for the Detection of Aflatoxin B1","abstract":null,"journal":"ACS Applied Materials & Interfaces","year":2021,"id":680786,"datarank":0.7301301675683375,"base_score":4.867534450455582,"endowment":4.867534450455582,"self_citation_contribution":0.7301301675683375,"citation_network_contribution":0.0,"self_endowment_contribution":0.7301301675683375,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":129,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":424155,"name":"Hao Jiang","orcid":"0000-0002-8845-4378","position":1,"is_corresponding":false},{"id":1778685,"name":"Guangyin Zhang","orcid":null,"position":2,"is_corresponding":false},{"id":1110961,"name":"Qian Luo","orcid":"0000-0002-3413-9727","position":3,"is_corresponding":false},{"id":586057,"name":"Qian Zhao","orcid":"0000-0002-4017-465X","position":4,"is_corresponding":false},{"id":1778686,"name":"Xingbo Shi","orcid":"0000-0001-5062-7424","position":5,"is_corresponding":false},{"id":734134,"name":"Dai Lu","orcid":"0000-0003-2630-3184","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"An In Situ Generated Prussian Blue Nanoparticle-Mediated Multimode Nanozyme-Linked Immunosorbent Assay for the Detection of Aflatoxin B1","abstract":"This work aims to develop a novel multimode (photothermal/colorimetric/fluorescent) nanozyme-linked immunosorbent assay (NLISA) based on the in situ generation of Prussian blue nanoparticles (PBNPs) on the surface of magnetic nanoparticles (MNPs). Being considered the most toxic among the mycotoxins, aflatoxin B1 (AFB1) was chosen as the proof-of-concept target. In this strategy, MNPs, on which an AFB1 aptamer was previously assembled via streptavidin-biotin linkage, are anchored to 96-well plates by AFB1 and antibody. In the presence of HCl and K 4 Fe(CN) 6, PBNPs formed in situ on the MNP surface, thereby achieving photothermal and colorimetric signal readout due to their photothermal effect and intrinsic peroxidase-like activity. Based on fluorescence quenching by MNPs, Cy5 fluorescence was recovered by the in situ generation of PBNPs to facilitate ultrasensitive fluorescence detection. Photothermal and colorimetric signals allow portable/visual point-of-care testing, and fluorescent signals enable accurate determination with a detection limit of 0.54 fg/mL, which is 6333 and 28 times lower than those of photothermal and colorimetric analyses, respectively. We expect that this proposed multimode NLISA can not only reduce the false-positive/negative rates through the multisignal crossdetection in AFB1 monitoring but also provide a universal way of sophisticated instrumentation-free, easy-to-use, cost-effective, and highly sensitive detection of other food hazards.","is_dataset_classified":null,"base_score":4.867534450455582,"endowment":4.867534450455582,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"34043909","pmcid":null,"openalex_id":"https://openalex.org/W3165609888","authors":[],"funders":[{"funder_name":"Natural Science Foundation of??Hunan Province","grant_id":"2019JJ40115","title":null},{"funder_name":"Natural Science Foundation of??Hunan Province","grant_id":"2020JJ5249","title":null},{"funder_name":"Natural Science Foundation of??Hunan Province","grant_id":"2020RC3050","title":null},{"funder_name":"China Postdoctoral Science Foundation","grant_id":"2018M642980","title":null},{"funder_name":"National Natural Science Foundation of China","grant_id":"31972155","title":null},{"funder_name":"National Natural Science Foundation of China","grant_id":"32001778","title":null},{"funder_name":"Training Program of Excellent Young Innovators of Changsha","grant_id":"kq1905017","title":null},{"funder_name":"Training Program of Excellent Young Innovators of Changsha","grant_id":"kq2009058","title":null}],"total_grants":8,"fwci":6.9471,"citation_percentile":0.98290193,"influential_citations":0,"citation_trend":[{"year":2021,"count":5},{"year":2022,"count":22},{"year":2023,"count":33},{"year":2024,"count":36},{"year":2025,"count":23},{"year":2026,"count":10}],"oa_status":"closed","license":"https://doi.org/10.15223/policy-029","oa_locations":[{"url":"https://pubs.acs.org/doi/pdf/10.1021/acsami.1c04751","host_type":"publisher"},{"url":"https://doi.org/10.1021/acsami.1c04751","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/34043909","host_type":"repository"}],"fields_of_study":["Advanced Nanomaterials in Catalysis","Advanced biosensing and bioanalysis techniques","Biosensors and Analytical Detection","Acetic Acid","Aflatoxin B1","Arachis","Biological Assay","Biosensing Techniques","Ferrocyanides","Food Contamination","Immunosorbents","Nanoparticles","Wine"],"mesh_terms":["Biological Assay","Ferrocyanides","Food Contamination","Immunosorbents","Arachis","Wine","Biosensing Techniques","Aflatoxin B1","Acetic Acid","Nanoparticles"],"keywords":["Prussian blue","Photothermal therapy","Detection limit","Fluorescence","Nanoparticle","Materials science","Aptamer","Streptavidin","Colorimetry","In situ","Nanotechnology","Biosensor","Aflatoxin","Photothermal effect","Multi-mode optical fiber","Magnetic nanoparticles","Chromatography","Chemistry","Optics","Biotin","Optical fiber","Organic chemistry","Aflatoxin B1","Point-of-care Testing","Prussian Blue Nanoparticles","Multimode Signals"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-17T16:05:09.745314Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}