{"doi":"10.1021/acsabm.2c00303","title":"Bioactive Fibronectin-III <sub>10</sub> –DNA Origami Nanofibers Promote Cell Adhesion and Spreading","abstract":"The integration of proteins with DNA nanotechnology would enable materials with diverse applications in biology, medicine, and engineering. Here, we describe a method for the incorporation of bioactive fibronectin domain proteins with DNA nanostructures using two orthogonal coiled-coil peptides. One peptide from each coiled-coil pair is attached to a DNA origami cuboid in a multivalent fashion by attaching the peptides to DNA handles. These structures can then be assembled into one-dimensional arrays through the addition of a fibronectin domain linker genetically fused with the complementary peptides to those on the origami. We validate array formation using two different self-assembly protocols and characterize the fibers by atomic force and electron microscopy. Finally, we demonstrate that surfaces coated with the protein-DNA nanofibers can serve as biomaterial substrates for fibroblast adhesion and spreading with the nanofibers showing enhanced bioactivity compared to that of the monomeric protein.","journal":"ACS Applied Bio Materials","year":2022,"id":257853,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":18,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9522,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":606958,"name":"Kyle D. Riker","orcid":"0000-0003-4788-8687","position":1,"is_corresponding":false},{"id":908381,"name":"Julio Bernal‐Chanchavac","orcid":"0000-0001-5093-4248","position":2,"is_corresponding":false},{"id":908382,"name":"Raghu Pradeep Narayanan","orcid":"0000-0002-7036-9706","position":3,"is_corresponding":false},{"id":820145,"name":"C.R. Simmons","orcid":"0000-0002-2290-6132","position":4,"is_corresponding":false},{"id":908383,"name":"Nour Eddine Fahmi","orcid":"0000-0001-6829-9756","position":5,"is_corresponding":false},{"id":353583,"name":"Ronit Freeman","orcid":"0000-0001-5960-6689","position":6,"is_corresponding":false},{"id":504881,"name":"Nicholas Stephanopoulos","orcid":"0000-0001-7859-410X","position":7,"is_corresponding":false},{"id":867011,"name":"Alex Buchberger","orcid":"0000-0003-3412-8945","position":0,"is_corresponding":true}],"reference_count":37,"raw_metadata":null,"created_at":"2026-07-19T00:25:34.440699Z","pmid":"36108278","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}