{"doi":"10.1021/acsabm.1c00890","title":"Polyethylenimine-Functionalized Carbon Dots for Delivery of CRISPR/Cas9 Complexes","abstract":"Carbon dots (CDs) have become the focus of many studies due to their outstanding optical properties and good biocompatibility. We investigated their potential application to produce a smart and highly efficient yet nontoxic nanovector for gene delivery. This was achieved by conjugating PEI1.8k-functionalized CDs (synthesized by one-step microwave-assisted pyrolysis) with arginine-disulfide linkers to produce CD-PEI1.8k-Arg nanoparticles. This nanovector could deliver p-CRISPR (9.3 kb) into different types of cell lines with higher efficiency compared to native PEI1.8k or PEI25k. CD-PEI1.8k-Arg also maintained its outstanding transfection efficiency at a high serum concentration and low p-CRISPR dose, compared to PEI25k, which was ineffective under those conditions. Additionally, CD-PEI1.8k-Arg could knock out the GFP gene with great efficiency by delivering the required components of CRISPR/Cas9, including a plasmid encoding Cas9, sgRNA targeting GFP, and Cas9/sgRNA ribonucleoproteins (RNPs) into the HEK 293T-GFP cells. Moreover, the nanoparticles showed potential for the local delivery of p-CRISPR into brain tissue. The remarkable properties of CD-PEI1.8k-Arg could enable the development of a safe, highly efficient gene-delivery nanovector for the treatment of various diseases in the near future.","journal":"ACS Applied Bio Materials","year":2021,"id":169613,"datarank":0.515098080672772,"base_score":3.4339872044851463,"endowment":3.4339872044851463,"self_citation_contribution":0.515098080672772,"citation_network_contribution":0.0,"self_endowment_contribution":0.515098080672772,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":30,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9493,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":701386,"name":"Akbar Hasanzadeh","orcid":null,"position":1,"is_corresponding":false},{"id":701387,"name":"Fatemeh Radmanesh","orcid":null,"position":2,"is_corresponding":false},{"id":701388,"name":"Beheshteh Khodadadi Chegeni","orcid":null,"position":3,"is_corresponding":false},{"id":700684,"name":"Elaheh Sadat Hosseini","orcid":"0000-0002-1420-0842","position":4,"is_corresponding":false},{"id":700685,"name":"Jafar Kiani","orcid":"0000-0002-5907-5846","position":5,"is_corresponding":false},{"id":700686,"name":"Ali Shahbazi","orcid":"0000-0001-5222-4792","position":6,"is_corresponding":false},{"id":700687,"name":"Marzieh Naseri","orcid":"0000-0003-0426-8680","position":7,"is_corresponding":false},{"id":240557,"name":"Yousef Fatahi","orcid":"0000-0003-0374-2141","position":8,"is_corresponding":false},{"id":700688,"name":"Helena Nourizadeh","orcid":"0000-0003-2129-5285","position":9,"is_corresponding":false},{"id":701389,"name":"Behjat Kheiri Yeghaneh Azar","orcid":null,"position":10,"is_corresponding":false},{"id":700689,"name":"Amir Reza Aref","orcid":"0000-0002-5174-0522","position":11,"is_corresponding":false},{"id":275945,"name":"Yong Liu","orcid":"0000-0003-1738-7857","position":12,"is_corresponding":false},{"id":240564,"name":"Michael R. Hamblin","orcid":"0000-0001-6431-4605","position":13,"is_corresponding":false},{"id":700690,"name":"Mahdi Karimi","orcid":"0000-0002-9358-9613","position":14,"is_corresponding":false},{"id":701385,"name":"Iman Hashemzadeh","orcid":null,"position":0,"is_corresponding":true}],"reference_count":59,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T23:46:15.560174Z","pmid":"35006779","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}