{"doi":"10.1021/acs.jproteome.4c00668","title":"Achieving a 35-Plex Tandem Mass Tag Reagent Set through Deuterium Incorporation","abstract":"Mass spectrometry-based sample multiplexing with isobaric tags permits the development of high-throughput and precise quantitative biological assays with proteome-wide coverage and minimal missing values. Here, we nearly doubled the multiplexing capability of the TMTpro reagent set to a 35-plex through the incorporation of one deuterium isotope into the reporter group. Substituting deuterium frequently results in suboptimal peak coelution, which can compromise the accuracy of reporter ion-based quantification. To counteract the deuterium effect on quantitation, we implemented a strategy that necessitated the segregation of nondeuterium and deuterium-containing channels into distinct subplexes during normalization procedures, with reassembly through a common bridge channel. This multiplexing strategy of \"design independent sub-plexes but acquire together\" (DISAT) was used to compare protein expression differences between human cell lines and in a cysteine-profiling (i.e., chemoproteomics) experiment to identify compounds binding to cysteine-113 of Pin1.","journal":"Journal of Proteome Research","year":2024,"id":418824,"datarank":0.586803450814222,"base_score":3.912023005428146,"endowment":3.912023005428146,"self_citation_contribution":0.586803450814222,"citation_network_contribution":0.0,"self_endowment_contribution":0.586803450814222,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":49,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9505,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":390946,"name":"Dustin C. Frost","orcid":"0000-0002-8239-5274","position":1,"is_corresponding":false},{"id":663100,"name":"Karsten Kuhn","orcid":"0000-0002-3323-7341","position":2,"is_corresponding":false},{"id":505671,"name":"Myungsun Shin","orcid":null,"position":3,"is_corresponding":false},{"id":1033002,"name":"Rebecca L. Whitehouse","orcid":"0000-0001-5809-8731","position":4,"is_corresponding":false},{"id":984563,"name":"Ting-Yu Wei","orcid":"0000-0002-3073-625X","position":5,"is_corresponding":false},{"id":109560,"name":"Yuchen He","orcid":"0000-0002-6096-4915","position":6,"is_corresponding":false},{"id":1033003,"name":"S L Dawson","orcid":"0000-0003-2212-0911","position":7,"is_corresponding":false},{"id":663106,"name":"Ian Pike","orcid":"0000-0002-3071-0041","position":8,"is_corresponding":false},{"id":89979,"name":"Ryan Bomgarden","orcid":null,"position":9,"is_corresponding":false},{"id":107041,"name":"Steven P. Gygi","orcid":"0000-0001-7626-0034","position":10,"is_corresponding":false},{"id":136624,"name":"Joao A. Paulo","orcid":"0000-0002-4291-413X","position":11,"is_corresponding":false},{"id":883618,"name":"Nathan R. Zuniga","orcid":"0000-0001-8718-0638","position":0,"is_corresponding":true}],"reference_count":41,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T01:57:09.067930Z","pmid":"39380184","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}