{"doi":"10.1021/acs.jproteome.4c00502","title":"LC-Orbitrap HRMS-Based Proteomics Reveals Novel Mitochondrial Dynamics Regulatory Proteins Associated with <i>Ras</i><i>V12-</i>Induced Glioblastoma (GBM) of <i>Drosophila</i>","abstract":null,"journal":"Journal of Proteome Research","year":2024,"id":635145,"datarank":0.10397207708399181,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"self_citation_contribution":0.10397207708399181,"citation_network_contribution":0.0,"self_endowment_contribution":0.10397207708399181,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1058635,"name":"Rohit Kumar","orcid":"0000-0001-5033-2810","position":1,"is_corresponding":false},{"id":975527,"name":"Prabhat Kumar","orcid":null,"position":2,"is_corresponding":false},{"id":1647714,"name":"Sunaina Kushwaha","orcid":null,"position":3,"is_corresponding":false},{"id":1647715,"name":"Sandhya Kumari","orcid":null,"position":4,"is_corresponding":false},{"id":1315865,"name":"Neha Yadav","orcid":"0009-0005-0459-6336","position":5,"is_corresponding":false},{"id":1647716,"name":"Saripella Srikrishna","orcid":"0000-0002-6019-1588","position":6,"is_corresponding":false},{"id":276856,"name":"Pradeep Kumar","orcid":"0000-0002-6545-9555","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"LC-Orbitrap HRMS-Based Proteomics Reveals Novel Mitochondrial Dynamics Regulatory Proteins Associated with <i>Ras</i><i>V12-</i>Induced Glioblastoma (GBM) of <i>Drosophila</i>","abstract":"Glioblastoma multiforme (GBM) is the most prevalent and aggressive brain tumor found in adult humans with a poor prognosis and average survival of 14–15 months. In order to have a comprehensive understanding of proteome and identify novel therapeutic targets, this study focused mainly on the differentially abundant proteins (DAPs) of Ras V12 -induced GBM. Ras V12 is a constitutively active Ras mutant form essential for tumor progression by continuously activating signaling pathways leading to uncontrolled tumor growth. This study used a transgenic Drosophila model with Ras V12 overexpression using the repo-GAL4 driver line, specifically in glial cells, to study GBM. The high-resolution mass spectrometry (HRMS)-based proteomic analysis of the GBM larval central nervous system identified three novel DAPs specific to mitochondria. These DAPs, probable maleylacetoacetate isomerase 2 (Q9VHD2), bifunctional methylene tetrahydrofolate dehydrogenase (Q04448), and glutamine synthetase1 (P20477), identified through HRMS were further validated by qRT-PCR. The protein–protein interaction analysis revealed interactions between Ras V12 and DAPs, with functional links to mitochondrial dynamics regulators such as Drp1, Marf, Parkin, and HtrA2. Notably, altered expressions of Q9VHD2, P20477, and Q04448 were observed during GBM progression, which offers new insights into the involvement of mitochondrial dynamic regulators in Ras V12 -induced GBM pathophysiology.","is_dataset_classified":null,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"39413821","pmcid":null,"openalex_id":"https://openalex.org/W4403458752","authors":[],"funders":[{"funder_name":"Banaras Hindu University","grant_id":"","title":null},{"funder_name":"Council of Scientific and Industrial Research, India","grant_id":"","title":null},{"funder_name":"University Grants Commission","grant_id":"","title":null}],"total_grants":3,"fwci":0.1662,"citation_percentile":0.50266393,"influential_citations":0,"citation_trend":[{"year":2026,"count":1}],"oa_status":"closed","license":"https://doi.org/10.15223/policy-029","oa_locations":[{"url":"https://pubs.acs.org/doi/pdf/10.1021/acs.jproteome.4c00502","host_type":"publisher"},{"url":"https://doi.org/10.1021/acs.jproteome.4c00502","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/39413821","host_type":"repository"}],"fields_of_study":["Mitochondrial Function and Pathology","Ubiquitin and proteasome pathways","Cancer, Hypoxia, and Metabolism","Glioblastoma","Animals","Proteomics","Mitochondrial Dynamics","Drosophila Proteins","Animals, Genetically Modified","Brain Neoplasms","Mass Spectrometry","Mitochondria","Humans","Chromatography, Liquid","Glutamate-Ammonia Ligase","Proteome","Drosophila melanogaster","Mitochondrial Proteins","Disease Models, Animal"],"mesh_terms":["Animals","Brain Neoplasms","Chromatography, Liquid","Disease Models, Animal","Drosophila melanogaster","Glioblastoma","Glutamate-Ammonia Ligase","Humans","Mitochondria","Mass Spectrometry","Proteome","Mitochondrial Proteins","Drosophila Proteins","Animals, Genetically Modified","Proteomics","Mitochondrial Dynamics"],"keywords":["Glioblastoma","Orbitrap","Proteomics","Quantitative proteomics","Biology","Chemistry","Computational biology","Cancer research","Biochemistry","Gene","Mass spectrometry","Chromatography","Drosophila","CNS","Brain tumor","Mitochondrial Dynamics","Rasv12"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Zero hunger"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-06T14:42:46.664225Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}