{"doi":"10.1021/acs.jproteome.3c00259","title":"Identification of LMAN1- and SURF4-Dependent Secretory Cargoes","abstract":null,"journal":"Journal of Proteome Research","year":2023,"id":663945,"datarank":0.459986156139158,"base_score":2.302585092994046,"endowment":2.302585092994046,"self_citation_contribution":0.3453877639491069,"citation_network_contribution":0.11459839219005108,"self_endowment_contribution":0.3453877639491069,"citer_contribution":0.11459839219005108,"corpus_percentile":null,"corpus_rank":null,"citation_count":9,"citer_count":5,"citers_with_citation_signal":4,"citers_with_endowment":4,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":824734,"name":"Prabhodh S. Abbineni","orcid":"0000-0002-9700-0044","position":1,"is_corresponding":false},{"id":51779,"name":"Felipe da Veiga Leprevost","orcid":"0000-0002-8228-5374","position":2,"is_corresponding":false},{"id":253569,"name":"Venkatesha Basrur","orcid":"0000-0002-0853-9655","position":3,"is_corresponding":false},{"id":499041,"name":"Rami Khoriaty","orcid":"0000-0003-2539-5953","position":4,"is_corresponding":false},{"id":499038,"name":"Brian T. Emmer","orcid":"0000-0001-7365-1021","position":5,"is_corresponding":false},{"id":51780,"name":"Alexey I. Nesvizhskii","orcid":"0000-0002-2806-7819","position":6,"is_corresponding":false},{"id":345469,"name":"David Ginsburg","orcid":"0000-0002-6436-8942","position":7,"is_corresponding":false},{"id":499039,"name":"Vi T. Tang","orcid":"0000-0001-6079-9756","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Identification of LMAN1- and SURF4-Dependent Secretory Cargoes","abstract":"Most proteins secreted into the extracellular space are first recruited from the endoplasmic reticulum into coat protein complex II (COPII)-coated vesicles or tubules that facilitate their transport to the Golgi apparatus. Although several secreted proteins have been shown to be actively recruited into COPII vesicles and tubules by the cargo receptors LMAN1 and SURF4, the full cargo repertoire of these receptors is unknown. We now report mass spectrometry analysis of conditioned media and cell lysates from HuH7 cells CRISPR targeted to inactivate the <i>LMAN1</i> or <i>SURF4</i> gene. We found that LMAN1 has limited clients in HuH7 cells, whereas SURF4 traffics a broad range of cargoes. Analysis of putative SURF4 cargoes suggests that cargo recognition is governed by complex mechanisms rather than interaction with a universal binding motif..","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"37844105","pmcid":"PMC10629478","openalex_id":null,"authors":[],"funders":[{"funder_name":"National Heart, Lung, and Blood Institute","grant_id":"K08HL148552","title":null},{"funder_name":"National Heart, Lung, and Blood Institute","grant_id":"R01HL148333","title":null},{"funder_name":"National Heart, Lung, and Blood Institute","grant_id":"R01HL157062","title":null},{"funder_name":"National Heart, Lung, and Blood Institute","grant_id":"R35HL135793","title":null},{"funder_name":"American Heart Association","grant_id":"20PRE35210706","title":null},{"funder_name":"National Institute of General Medical Sciences","grant_id":"K99GM141268","title":null},{"funder_name":"National Institute of General Medical Sciences","grant_id":"R01GM094231","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R00 GM141268","title":null},{"funder_name":"Howard Hughes Medical Institute","grant_id":"","title":null},{"funder_name":"Horace H. Rackham School of Graduate Studies, University of Michigan","grant_id":"","title":null}],"total_grants":10,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":"hybrid","license":"cc-by-nc-nd","oa_locations":[{"url":"https://pubs.acs.org/doi/pdf/10.1021/acs.jproteome.3c00259","host_type":"publisher"},{"url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC10629478/pdf/pr3c00259.pdf","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC10629478","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC10629478?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":[],"mesh_terms":["Endoplasmic Reticulum","Golgi Apparatus","Humans","Carrier Proteins","Membrane Proteins","Protein Transport"],"keywords":["Secretome","Cargo Receptor","Copii Trafficking"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-13T00:18:20.987124Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}