{"doi":"10.1021/acs.jpclett.3c01040","title":"Stability of 20S Proteasome Configurations: Preopening the Axial Gate","abstract":"20S proteasome from 11 to 55 °C reveal a series of related configurations and coupled transitions that appear to be associated with opening of the proteolytic core. We find no evidence for dissociation, and all transitions are reversible. A thermodynamic analysis indicates that configurations fall into three general types of structures: enthalpically stabilized, tightly closed (observed as the +54 to +58 charge states) configurations; high-entropy (+60 to +66) states that are proposed as precursors to pore opening; and larger (+70 to +79) partially and fully open pore structures. In the absence of the 19S regulatory unit, the mechanism for opening the 20S pore appears to involve a charge-priming process that loosens the closed-pore configuration. Only a small fraction (≤2%) of these 20S precursor configurations appear to open and thus expose the catalytic cavity.","journal":"The Journal of Physical Chemistry Letters","year":2023,"id":351180,"datarank":0.46505235220155694,"base_score":2.302585092994046,"endowment":2.302585092994046,"self_citation_contribution":0.3453877639491069,"citation_network_contribution":0.11966458825245004,"self_endowment_contribution":0.3453877639491069,"citer_contribution":0.11966458825245004,"corpus_percentile":null,"corpus_rank":null,"citation_count":9,"citer_count":5,"citers_with_citation_signal":4,"citers_with_endowment":4,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9532,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1084088,"name":"Edie M. Sharon","orcid":null,"position":1,"is_corresponding":false},{"id":781365,"name":"Amit Kumar Singh Gautam","orcid":"0000-0001-9846-1945","position":2,"is_corresponding":false},{"id":1041048,"name":"Adam Anthony","orcid":null,"position":3,"is_corresponding":false},{"id":490066,"name":"Martin F. Jarrold","orcid":"0000-0001-7084-176X","position":4,"is_corresponding":false},{"id":323307,"name":"David H. Russell","orcid":"0000-0003-0830-3914","position":5,"is_corresponding":false},{"id":253739,"name":"Andreas Matouschek","orcid":"0000-0001-6016-2341","position":6,"is_corresponding":false},{"id":244927,"name":"David E. Clemmer","orcid":"0000-0003-4039-1360","position":7,"is_corresponding":false},{"id":1084089,"name":"Lucas W. Henderson","orcid":null,"position":0,"is_corresponding":true}],"reference_count":25,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T01:12:41.740237Z","pmid":"37224454","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}