{"doi":"10.1021/acs.jpcb.1c07762","title":"A Comparative Study of High-Contrast Fluorescence Lifetime Probes for Imaging Amyloid in Tissue","abstract":null,"journal":"The Journal of Physical Chemistry B","year":2021,"id":628584,"datarank":0.3453877639491069,"base_score":2.302585092994046,"endowment":2.302585092994046,"self_citation_contribution":0.3453877639491069,"citation_network_contribution":0.0,"self_endowment_contribution":0.3453877639491069,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":9,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1627500,"name":"Sam Daly","orcid":null,"position":1,"is_corresponding":false},{"id":496399,"name":"Colin M. 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This is applied specifically to imaging recombinant α-synuclein aggregates doped into brain tissue. Despite the 100-fold lower brightness of PAP_1 compared to that of Thioflavin T, PAP_1 binds to α-synuclein aggregates with an affinity several orders of magnitude greater than Thioflavin T; thus, we observe a specific decrease in the fluorescence lifetime of PAP_1 bound to α-synuclein aggregates, resulting in a separation of >1.4 standard deviations between PAP_1-stained brain tissue background and α-synuclein aggregates that is not observed with Thioflavin T. This enables contrast between highly fluorescent background tissue and amyloid fibrils that is attributed to the greater affinity of PAP_1 for α-synuclein aggregates, avoiding the substantial off-target staining observed with Thioflavin T.","is_dataset_classified":null,"base_score":2.302585092994046,"endowment":2.302585092994046,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"34883017","pmcid":"PMC7615715","openalex_id":"https://openalex.org/W4200202434","authors":[],"funders":[{"funder_name":"Royal Society","grant_id":"UF120277","title":null},{"funder_name":"Simons Foundation Autism Research Initiative","grant_id":"529085","title":null},{"funder_name":"H2020 Marie Sk??odowska-Curie Actions","grant_id":"886216","title":"Sizing Up PD: Cavity-enhanced Characterisation of Single Biomarkers in Human Biofluids"},{"funder_name":"Wellcome Trust","grant_id":"202932","title":"Molecular synapse imaging technology and resources"},{"funder_name":"Wellcome Trust","grant_id":"218293","title":"The Human Brain Synaptome Project"},{"funder_name":"National Institute of General Medical Sciences","grant_id":"R01GM121573","title":null},{"funder_name":"H2020 European Research Council","grant_id":"695568","title":"Molecular organization and dynamics of synapse diversity:  novel genetic, imaging and computational approaches"},{"funder_name":"National Institutes of Health","grant_id":"5R01GM121573-03","title":"Asymmetric Chemical Synthesis Methods via Cooperative Catalysis"},{"funder_name":"Wellcome Trust","grant_id":"unidentified","title":"unidentified"},{"funder_name":"Engineering and Physical Sciences Research Council","grant_id":"","title":null},{"funder_name":"Michael J. Fox Foundation for Parkinson's Research","grant_id":"","title":null}],"total_grants":11,"fwci":0.6853,"citation_percentile":0.68195609,"influential_citations":0,"citation_trend":[{"year":2023,"count":4},{"year":2024,"count":2},{"year":2026,"count":3}],"oa_status":"green","license":"other-oa","oa_locations":[{"url":"https://www.research.ed.ac.uk/en/publications/4ee7fd51-8f28-4c34-8826-cbbd89567a8e","host_type":"repository"},{"url":"https://www.research.ed.ac.uk/en/publications/4ee7fd51-8f28-4c34-8826-cbbd89567a8e","host_type":"repository"},{"url":"https://pubs.acs.org/doi/pdf/10.1021/acs.jpcb.1c07762","host_type":"publisher"},{"url":"https://doi.org/10.1021/acs.jpcb.1c07762","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/34883017","host_type":"repository"},{"url":"https://www.repository.cam.ac.uk/handle/1810/346472.2","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/7615715","host_type":"repository"},{"url":"https://www.repository.cam.ac.uk/handle/1810/346472","host_type":"repository"},{"url":"https://doi.org/10.17863/cam.93892","host_type":"repository"},{"url":"https://www.repository.cam.ac.uk/bitstreams/545457ca-788d-48a5-bb96-80342e7be8e1/download","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC7615715","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC7615715?pdf=render","host_type":"Europe_PMC"},{"url":"https://dx.doi.org/10.17863/cam.93892","host_type":""},{"url":"http://dx.doi.org/10.1021/acs.jpcb.1c07762","host_type":""},{"url":"http://dx.doi.org/10.1021/acs.jpclett.0c01549","host_type":""},{"url":"https://zenodo.org/records/7587474","host_type":""},{"url":"https://pubs.acs.org/doi/10.1021/acs.jpcb.1c07762","host_type":""},{"url":"https://hdl.handle.net/20.500.11820/4ee7fd51-8f28-4c34-8826-cbbd89567a8e","host_type":""},{"url":"https://www.pure.ed.ac.uk/ws/files/248515310/Gorka_PAP1AmyloidFLIM_MS_Accepted.pdf","host_type":""}],"fields_of_study":["Alzheimer's disease research and treatments","Parkinson's Disease Mechanisms and Treatments","Dementia and Cognitive Impairment Research","0301 basic medicine","0303 health sciences","03 medical and health sciences","Amyloid","Amyloid beta-Peptides","Amyloidogenic Proteins","Benzothiazoles","Fluorescent Dyes","Optical Imaging","Spectrometry, Fluorescence","alpha-Synuclein"],"mesh_terms":["Amyloid","Fluorescent Dyes","Spectrometry, Fluorescence","Amyloid beta-Peptides","alpha-Synuclein","Benzothiazoles","Amyloidogenic Proteins","Optical Imaging"],"keywords":["Thioflavin","Fluorescence","Amyloid (mycology)","Biophysics","Chemistry","Fluorescence-lifetime imaging microscopy","Pathology","Biology","Alzheimer's disease","Optics","Medicine","Amyloid","Amyloid beta-Peptides","Spectrometry, Fluorescence","Optical Imaging","alpha-Synuclein","Amyloidogenic Proteins","Benzothiazoles","Article","Fluorescent Dyes"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"pdb"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-05T14:09:57.803091Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}