{"doi":"10.1021/acs.jnatprod.3c00151","title":"The Sea Cucumber <i>Thyonella gemmata</i> Contains a Low Anticoagulant Sulfated Fucan with High Anti-SARS-CoV-2 Actions against Wild-Type and Delta Variants","abstract":"In this work, we isolated two new sulfated glycans from the body wall of the sea cucumber Thyonella gemmata: one fucosylated chondroitin sulfate (TgFucCS) (17.5 ± 3.5% kDa) and one sulfated fucan (TgSF) (383.3 ± 2.1% kDa). NMR results showed the TgFucCS backbone composed of [→3)-β- N -acetylgalactosamine-(1→4)-β-glucuronic acid-(1→] with 70% 4-sulfated and 30% 4,6-disulfated GalNAc units and one-third of the GlcA units decorated at the C3 position with branching α-fucose (Fuc) units either 4-sulfated (65%) or 2,4-disulfated (35%) and the TgSF structure composed of a tetrasaccharide repeating unit of [→3)-α-Fuc2,4S-(1→2)-α-Fuc4S-(1→3)-α-Fuc2S-(1→3)-α-Fuc2S-(1→] n . Inhibitory properties of TgFucCS and TgSF were investigated using SARS-CoV-2 pseudovirus coated with S-proteins of the wild-type (Wuhan-Hu-1) or the delta (B.1.617.2) strains and in four different anticoagulant assays, comparatively with unfractionated heparin. Molecular binding to coagulation (co)-factors and S-proteins was investigated by competitive surface plasmon resonance spectroscopy. Among the two sulfated glycans tested, TgSF showed significant anti-SARS-CoV-2 activity against both strains together with low anticoagulant properties, indicating a good candidate for future studies in drug development.","journal":"Journal of Natural Products","year":2023,"id":347933,"datarank":0.37273599746820013,"base_score":2.4849066497880004,"endowment":2.4849066497880004,"self_citation_contribution":0.37273599746820013,"citation_network_contribution":0.0,"self_endowment_contribution":0.37273599746820013,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":11,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9526,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":915281,"name":"Marwa Farrag","orcid":"0000-0001-7104-3815","position":1,"is_corresponding":false},{"id":300828,"name":"Poonam Sharma","orcid":"0000-0003-4488-2781","position":2,"is_corresponding":false},{"id":860513,"name":"Deling Shi","orcid":"0000-0002-3404-2538","position":3,"is_corresponding":false},{"id":956310,"name":"Anter A. Shami","orcid":null,"position":4,"is_corresponding":false},{"id":476781,"name":"Sandeep K. Misra","orcid":"0000-0001-9165-3987","position":5,"is_corresponding":false},{"id":1090212,"name":"Priya Ray","orcid":null,"position":6,"is_corresponding":false},{"id":1090213,"name":"Jayanti Shukla","orcid":null,"position":7,"is_corresponding":false},{"id":233177,"name":"Fuming Zhang","orcid":"0000-0003-2803-3704","position":8,"is_corresponding":false},{"id":286697,"name":"Robert J. Linhardt","orcid":"0000-0003-2219-5833","position":9,"is_corresponding":false},{"id":476783,"name":"Joshua S. Sharp","orcid":"0000-0002-0115-0276","position":10,"is_corresponding":false},{"id":300829,"name":"Ritesh Tandon","orcid":"0000-0001-8996-8840","position":11,"is_corresponding":false},{"id":789006,"name":"Vitor H. Pomin","orcid":"0000-0002-5015-8880","position":12,"is_corresponding":false},{"id":78942,"name":"Rohini Dwivedi","orcid":"0000-0001-6330-3664","position":0,"is_corresponding":true}],"reference_count":47,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T01:12:01.859977Z","pmid":"37306476","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}