{"doi":"10.1021/acs.jafc.3c01946","title":"Engineered Yeast Displaying Specific Norovirus-Binding Nanobodies for the Concentration and Detection of Human Norovirus in Food Matrix","abstract":"Human noroviruses pose grave threats to public health and economy. In this study, we genetically engineered yeast ( Saccharomyces cerevisiae EBY100) to display specific norovirus-binding nanobodies (Nano-26 and Nano-85) on cell surface to facilitate the concentration of noroviruses for improved detection. Binding of norovirus virus-like particles (VLPs) to these nanobody-displaying yeasts was confirmed and characterized using confocal microscopy and flow cytometry. The ability of our engineered yeasts to capture norovirus VLPs can reach up to 91.3%. Furthermore, this approach was applied to concentrate and detect norovirus VLPs in a real food matrix. A wide linear detection range (1–10 4 pg/g) was observed, and the detection limit on spiked spinach was calculated as low as 0.071 pg/g. Overall, our engineered yeasts could be a promising approach to concentrate and purify noroviruses in food samples for easy detection, which allows us to prevent the spread of food-borne virus in the food supply chain.","journal":"Journal of Agricultural and Food Chemistry","year":2023,"id":349549,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":10,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9526,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":328644,"name":"Mahbubur Rahman","orcid":"0000-0003-0520-2683","position":1,"is_corresponding":false},{"id":1048891,"name":"Zhiyuan Xu","orcid":"0000-0002-6352-2629","position":2,"is_corresponding":false},{"id":1029301,"name":"Tom Kasputis","orcid":"0000-0003-0772-0599","position":3,"is_corresponding":false},{"id":1029299,"name":"Yawen He","orcid":"0009-0001-4898-5465","position":4,"is_corresponding":false},{"id":690619,"name":"Lijuan Yuan","orcid":"0000-0003-0709-5228","position":5,"is_corresponding":false},{"id":808108,"name":"R Clay Wright","orcid":"0000-0001-7125-3943","position":6,"is_corresponding":false},{"id":1029302,"name":"Juhong Chen","orcid":"0000-0002-6484-2739","position":7,"is_corresponding":false},{"id":1048892,"name":"Xue Zhao","orcid":"0000-0002-6337-8137","position":0,"is_corresponding":true}],"reference_count":52,"raw_metadata":null,"created_at":"2026-07-19T01:12:19.059218Z","pmid":"37227100","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}