{"doi":"10.1021/acs.jafc.2c07613","title":"Integrating Enzyme Evolution and Metabolic Engineering to Improve the Productivity of Γ-Aminobutyric Acid by Whole-Cell Biosynthesis in <i>Escherichia Coli</i>","abstract":null,"journal":"Journal of Agricultural and Food Chemistry","year":2023,"id":674426,"datarank":0.4335557636844247,"base_score":2.8903717578961645,"endowment":2.8903717578961645,"self_citation_contribution":0.4335557636844247,"citation_network_contribution":0.0,"self_endowment_contribution":0.4335557636844247,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":17,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1762111,"name":"Xiaojing Huo","orcid":null,"position":1,"is_corresponding":false},{"id":1762112,"name":"Yaqian Tang","orcid":null,"position":2,"is_corresponding":false},{"id":1155761,"name":"Mingyue Zhao","orcid":"0009-0008-8235-3200","position":3,"is_corresponding":false},{"id":39225,"name":"Yong Tao","orcid":"0000-0003-1443-2667","position":4,"is_corresponding":false},{"id":1208916,"name":"Jianzhong Huang","orcid":"0000-0002-0201-9130","position":5,"is_corresponding":false},{"id":1762113,"name":"Chongrong Ke","orcid":null,"position":6,"is_corresponding":false},{"id":1762110,"name":"Xinwei Yang","orcid":"0000-0003-0776-8269","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Integrating Enzyme Evolution and Metabolic Engineering to Improve the Productivity of Γ-Aminobutyric Acid by Whole-Cell Biosynthesis in <i>Escherichia Coli</i>","abstract":"γ-Aminobutyric acid (GABA) is used widely in various fields, such as agriculture, food, pharmaceuticals, and biobased chemicals. Based on glutamate decarboxylase (GadBM4) derived from our previous work, three mutants, GadM4-2, GadM4-8, and GadM4-31, were obtained by integrating enzyme evolution and high-throughput screening methods. The GABA productivity obtained through whole-cell bioconversion using recombinant Escherichia coli cells harboring mutant GadBM4-2 was enhanced by 20.27% compared to that of the original GadBM4. Further introduction of the central regulator GadE of the acid resistance system and the enzymes from the deoxyxylulose-5-phosphate-independent pyridoxal 5′-phosphate biosynthesis pathway resulted in a 24.92% improvement in GABA productivity, reaching 76.70 g/L/h without any cofactor addition with a greater than 99% conversion ratio. Finally, when one-step bioconversion was applied for the whole-cell catalysis in a 5 L bioreactor, the titer of GABA reached 307.5 ± 5.94 g/L with a productivity of 61.49 g/L/h by using crude l -glutamic acid ( l -Glu) as the substrate. Thus, the biocatalyst constructed above combined with the whole-cell bioconversion method represents an effective approach for industrial GABA production.","is_dataset_classified":null,"base_score":2.8903717578961645,"endowment":2.8903717578961645,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"36881553","pmcid":null,"openalex_id":"https://openalex.org/W4323348652","authors":[],"funders":[{"funder_name":"Natural Science Foundation of Fujian Province","grant_id":"2021J01170","title":null},{"funder_name":"Natural Science Foundation of Fujian Province","grant_id":"2021J01172","title":null}],"total_grants":2,"fwci":4.5962,"citation_percentile":0.93817927,"influential_citations":0,"citation_trend":[{"year":2023,"count":2},{"year":2024,"count":6},{"year":2025,"count":3},{"year":2026,"count":5}],"oa_status":"closed","license":"https://doi.org/10.15223/policy-029","oa_locations":[{"url":"https://pubs.acs.org/doi/pdf/10.1021/acs.jafc.2c07613","host_type":"publisher"},{"url":"https://doi.org/10.1021/acs.jafc.2c07613","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/36881553","host_type":"repository"}],"fields_of_study":["GABA and Rice Research","Microbial Metabolic Engineering and Bioproduction","Probiotics and Fermented Foods","Escherichia coli","Metabolic Engineering","Glutamate Decarboxylase","gamma-Aminobutyric Acid","Glutamic Acid","Phosphates"],"mesh_terms":["Escherichia coli","gamma-Aminobutyric Acid","Glutamate Decarboxylase","Phosphates","Glutamic Acid","Metabolic Engineering"],"keywords":["Biosynthesis","Escherichia coli","Metabolic engineering","Aminobutyric acid","Biochemistry","Enzyme","Productivity","Chemistry","Biology","Gene","Gamma-aminobutyric acid","Enzyme Evolution","Whole-cell Bioconversion"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Zero hunger"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-16T16:49:58.229023Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}