{"doi":"10.1021/acs.biochem.4c00703","title":"Identification of the Polymerizing Glycosyltransferase Required for the Addition of <scp>d</scp>-Glucuronic Acid to the Capsular Polysaccharide of <i>Campylobacter jejuni</i>","abstract":"High Resolution Image Download MS PowerPoint Slide Campylobacter jejuni is the leading cause of food poisoning in Europe and North America. The exterior surface of this bacterium is encased by a capsular polysaccharide that is attached to a diacyl glycerol phosphate anchor via a poly-Kdo (3-deoxy- d - manno -oct-2-ulosinic acid) linker. In the HS:2 serotype of C. jejuni NCTC 11168, the repeating trisaccharide consists of d -ribose, N -acetyl- d -glucosamine, and d -glucuronate. Here, we show that the N-terminal domain of Cj1432 (residues 1–356) is responsible for the reaction of the C2 hydroxyl group from the terminal d -ribose moiety of the growing polysaccharide chain with UDP- d -glucuronate as the donor substrate. This discovery represents the first biochemical identification and functional characterization of a glycosyltransferase responsible for the polymerization of the capsular polysaccharide of C. jejuni . The product of the reaction catalyzed by the N-terminal domain of Cj1432 is the substrate for the reaction catalyzed by the C-terminal domain of Cj1438 (residues 453–776). This enzyme catalyzes amide bond formation using the C6 carboxylate of the terminal d -glucuronate moiety and ( S )-serinol phosphate as substrates. It is also shown that Cj1435 catalyzes the hydrolysis of phosphate from the product catalyzed by the C-terminal domain of Cj1438. These results demonstrate that amide decoration of the d -glucuronate moiety occurs after the incorporation of this sugar into the growing polysaccharide chain.","journal":"Biochemistry","year":2025,"id":537902,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9598,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1424129,"name":"A.S. Riegert","orcid":"0000-0003-1846-3436","position":1,"is_corresponding":false},{"id":115238,"name":"Tamari Narindoshvili","orcid":null,"position":2,"is_corresponding":false},{"id":555543,"name":"Frank M. Raushel","orcid":"0000-0002-5918-3089","position":3,"is_corresponding":false},{"id":1118536,"name":"Dao Feng Xiang","orcid":null,"position":0,"is_corresponding":true}],"reference_count":48,"raw_metadata":null,"created_at":"2026-07-19T02:52:16.891390Z","pmid":"39854648","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}