{"doi":"10.1021/acs.biochem.3c00664","title":"Biosynthesis of UDP-α-<i>N</i>-Acetyl-<scp>d</scp><i>-</i>mannosaminuronic Acid and CMP-β-<i>N</i>-Acetyl-<scp>d</scp>-neuraminic Acid for the Capsular Polysaccharides of <i>Campylobacter jejuni</i>","abstract":"High Resolution Image Download MS PowerPoint Slide Campylobacter jejuni is a human pathogen and a leading cause of food poisoning in North America and Europe. The exterior surface of the bacterial cell wall is attached to a polymeric coat of sugar molecules known as the capsular polysaccharide (CPS) that helps protect the organism from the host immune response. The CPS is composed of a repeating sequence of common and unusual sugar residues. In the HS:11 serotype of C. jejuni, we identified two enzymes in the gene cluster for CPS formation that are utilized for the biosynthesis of UDP-α- N -acetyl- d -mannosaminuronic acid (UDP-ManNAcA). In the first step, UDP-α- N -acetyl- d -glucosamine (UDP-GlcNAc) is epimerized at C2 to form UDP-α- N -acetyl- d -mannosamine (UDP-ManNAc). This product is then oxidized by a NAD + -dependent C6-dehydrogenase to form UDP-ManNAcA. In the HS:6 serotype ( C. jejuni strain 81116), we identified three enzymes that are required for the biosynthesis of CMP-β- N -acetyl- d -neuraminic acid (CMP-Neu5Ac). In the first step, UDP-GlcNAc is epimerized at C2 and subsequently hydrolyzed to form N -acetyl- d -mannosamine (ManNAc) with the release of UDP. This product is then condensed with PEP by N -acetyl- d -neuraminate synthase to form N -acetyl- d -neuraminic acid (Neu5Ac). In the final step, CMP- N -acetyl- d -neuraminic acid synthase utilizes CTP to convert this product into CMP-Neu5Ac. A bioinformatic analysis of these five enzymes from C. jejuni serotypes HS:11 and HS:6 identified other bacterial species that can produce UDP-ManNAcA or CMP-Neu5Ac for CPS formation.","journal":"Biochemistry","year":2024,"id":479513,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9537,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":555543,"name":"Frank M. Raushel","orcid":"0000-0002-5918-3089","position":1,"is_corresponding":false},{"id":585204,"name":"Manas K. Ghosh","orcid":null,"position":0,"is_corresponding":true}],"reference_count":62,"raw_metadata":null,"created_at":"2026-07-19T02:06:54.284166Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}