{"doi":"10.1021/acs.biochem.3c00012","title":"Biosynthesis of 3,6-Dideoxy-heptoses for the Capsular Polysaccharides of <i>Campylobacter jejuni</i>","abstract":"Campylobacter jejuni is the leading cause of food poisoning in the United States. Surrounding the exterior surface of this bacterium is a capsular polysaccharide (CPS) that helps protect the organism from the host immune system. The CPS is composed of a repeating sequence of common and unusual sugar residues, including relatively rare heptoses. In the HS:5 serotype, we identified four enzymes required for the biosynthesis of GDP-3,6-dideoxy-β- l - ribo -heptose. In the first step, GDP- d - glycero -α- d - manno -heptose is dehydrated to form GDP-6-deoxy-4-keto-α- d - lyxo -heptose. This product is then dehydrated by a pyridoxal phosphate-dependent C3-dehydratase to form GDP-3,6-dideoxy-4-keto-α- d - threo -heptose before being epimerized at C5 to generate GDP-3,6-dideoxy-4-keto-β- l - erythro -heptose. In the final step, a C4-reductase uses NADPH to convert this product to GDP-3,6-dideoxy-β- l - ribo -heptose. These results are at variance with the previous report of 3,6-dideoxy- d - ribo -heptose in the CPS from serotype HS:5 of C. jejuni . We also demonstrated that GDP-3,6-dideoxy-β- l - xylo -heptose is formed using the corresponding enzymes found in the gene cluster from serotype HS:11 of C. jejuni . The utilization of different C4-reductases from other serotypes of C. jejuni enabled the formation of GDP-3,6-dideoxy-α- d - arabino -heptose and GDP-3,6-dideoxy-α- d - lyxo -heptose.","journal":"Biochemistry","year":2023,"id":363695,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":7,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9544,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1118536,"name":"Dao Feng Xiang","orcid":null,"position":1,"is_corresponding":false},{"id":555543,"name":"Frank M. Raushel","orcid":"0000-0002-5918-3089","position":2,"is_corresponding":false},{"id":585204,"name":"Manas K. Ghosh","orcid":null,"position":0,"is_corresponding":true}],"reference_count":25,"raw_metadata":null,"created_at":"2026-07-19T01:14:32.510714Z","pmid":"36943186","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}