{"doi":"10.1021/acs.analchem.8b00892","title":"Imaging of Lipids in Native Human Bone Sections Using TOF–Secondary Ion Mass Spectrometry, Atmospheric Pressure Scanning Microprobe Matrix-Assisted Laser Desorption/Ionization Orbitrap Mass Spectrometry, and Orbitrap–Secondary Ion Mass Spectrometry","abstract":null,"journal":"Analytical Chemistry","year":2018,"id":601633,"datarank":0.5955437870328184,"base_score":3.970291913552122,"endowment":3.970291913552122,"self_citation_contribution":0.5955437870328184,"citation_network_contribution":0.0,"self_endowment_contribution":0.5955437870328184,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":52,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1542699,"name":"Dhaka R. Bhandari","orcid":null,"position":1,"is_corresponding":false},{"id":1542701,"name":"Janina Werner","orcid":null,"position":2,"is_corresponding":false},{"id":1542702,"name":"Anja Henss","orcid":null,"position":3,"is_corresponding":false},{"id":1542703,"name":"Alexander Pirkl","orcid":null,"position":4,"is_corresponding":false},{"id":1542704,"name":"Matthias Kleine-Boymann","orcid":null,"position":5,"is_corresponding":false},{"id":1542705,"name":"Marcus Rohnke","orcid":"0000-0002-8867-950X","position":6,"is_corresponding":false},{"id":1542706,"name":"Sabine Wenisch","orcid":null,"position":7,"is_corresponding":false},{"id":445006,"name":"Elena Neumann","orcid":"0000-0001-7609-5964","position":8,"is_corresponding":false},{"id":1542707,"name":"Jürgen Janek","orcid":"0000-0002-9221-4756","position":9,"is_corresponding":false},{"id":1238503,"name":"Bernhard Spengler","orcid":"0000-0003-0179-5653","position":10,"is_corresponding":false},{"id":1542698,"name":"Kaija Schaepe","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Imaging of Lipids in Native Human Bone Sections Using TOF–Secondary Ion Mass Spectrometry, Atmospheric Pressure Scanning Microprobe Matrix-Assisted Laser Desorption/Ionization Orbitrap Mass Spectrometry, and Orbitrap–Secondary Ion Mass Spectrometry","abstract":"A method is described for high-resolution label-free molecular imaging of human bone tissue. To preserve the lipid content and the heterogeneous structure of osseous tissue, 4 μm thick human bone sections were prepared via cryoembedding and tape-assisted cryosectioning, circumventing the application of organic solvents and a decalcification step. A protocol for comparative mass spectrometry imaging (MSI) on the same section was established for initial analysis with time-of-flight secondary ion mass spectrometry (TOF-SIMS) at a lateral resolution of 10 μm to <500 nm, followed by atmospheric pressure scanning microprobe matrix-assisted laser desorption/ionization (AP-SMALDI) Orbitrap MSI at a lateral resolution of 10 μm. This procedure ultimately enabled MSI of lipids, providing the lateral localization of major lipid classes such as glycero-, glycerophospho-, and sphingolipids. Additionally, the applicability of the recently emerged Orbitrap-TOF-SIMS hybrid system was exemplarily examined and compared to the before-mentioned MSI methods.","is_dataset_classified":null,"base_score":3.970291913552122,"endowment":3.970291913552122,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"29944823","pmcid":null,"openalex_id":"https://openalex.org/W2810650971","authors":[],"funders":[{"funder_name":"Deutsche Forschungsgemeinschaft","grant_id":"SFB TRR 79","title":null},{"funder_name":"Deutsche Forschungsgemeinschaft","grant_id":"Sp 314/13-1","title":null},{"funder_name":"Deutsche Forschungsgemeinschaft","grant_id":"unidentified","title":"unidentified"}],"total_grants":3,"fwci":3.9145,"citation_percentile":0.94730763,"influential_citations":0,"citation_trend":[{"year":2019,"count":8},{"year":2020,"count":9},{"year":2021,"count":5},{"year":2022,"count":8},{"year":2023,"count":6},{"year":2024,"count":5},{"year":2025,"count":5},{"year":2026,"count":6}],"oa_status":"closed","license":null,"oa_locations":[{"url":"https://pubs.acs.org/doi/pdf/10.1021/acs.analchem.8b00892","host_type":"publisher"},{"url":"https://doi.org/10.1021/acs.analchem.8b00892","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/29944823","host_type":"repository"},{"url":"https://dx.doi.org/10.1021/acs.analchem.8b00892","host_type":""}],"fields_of_study":["Ion-surface interactions and analysis","Mass Spectrometry Techniques and Applications","Nuclear Physics and Applications","0301 basic medicine","03 medical and health sciences","01 natural sciences","0104 chemical sciences","Cryoultramicrotomy","Femur Head","Humans","Lipids","Optical Imaging","Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization","Spectrometry, Mass, Secondary Ion"],"mesh_terms":["Femur Head","Humans","Lipids","Cryoultramicrotomy","Spectrometry, Mass, Secondary Ion","Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization","Optical Imaging"],"keywords":["Orbitrap","Chemistry","Mass spectrometry","Mass spectrometry imaging","Thermal ionization mass spectrometry","Microprobe","Analytical Chemistry (journal)","Ion-mobility spectrometry–mass spectrometry","Atmospheric-pressure laser ionization","Ion","Sample preparation in mass spectrometry","Ion source","Chromatography","Ionization","Photoionization","Electrospray ionization","Mineralogy","Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization","Optical Imaging","Humans","Spectrometry, Mass, Secondary Ion","Femur Head","Lipids","Cryoultramicrotomy"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-29T16:57:06.789856Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}