{"doi":"10.1021/acs.analchem.7b02447","title":"Sensitive and Quantitative Detection of Anti-Poly(ethylene glycol) (PEG) Antibodies by Methoxy-PEG-Coated Surface Plasmon Resonance Sensors","abstract":null,"journal":"Analytical Chemistry","year":2017,"id":658905,"datarank":0.5333022092234121,"base_score":3.5553480614894135,"endowment":3.5553480614894135,"self_citation_contribution":0.5333022092234121,"citation_network_contribution":0.0,"self_endowment_contribution":0.5333022092234121,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":34,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1720074,"name":"Fang Sun","orcid":null,"position":1,"is_corresponding":false},{"id":1720075,"name":"Hsiang-Chieh Hung","orcid":null,"position":2,"is_corresponding":false},{"id":307086,"name":"Priyesh Jain","orcid":"0000-0002-1184-3148","position":3,"is_corresponding":false},{"id":1720076,"name":"Kasey Joanne Leger","orcid":null,"position":4,"is_corresponding":false},{"id":483151,"name":"Shaoyi Jiang","orcid":"0000-0001-9863-6899","position":5,"is_corresponding":false},{"id":838997,"name":"Peng Zhang","orcid":"0000-0002-5409-7480","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Sensitive and Quantitative Detection of Anti-Poly(ethylene glycol) (PEG) Antibodies by Methoxy-PEG-Coated Surface Plasmon Resonance Sensors","abstract":"Pre-existing and induced anti-poly(ethylene glycol) (PEG) antibodies (abs) have been shown to be related with limitation of therapeutic efficacy and reduction in tolerance of several therapeutic agents. However, the current methods to detect anti-PEG abs are tedious and usually lack quantification. A facile, rapid, sensitive, and reliable technique to detect anti-PEG abs is highly desired in both research and clinic settings. In this work, we have presented a surface plasmon resonance (SPR) biosensor technique for the detection of anti-PEG abs and compared three PEG surface chemistries. Methoxy-PEG (mPEG) 5k was found to have the best performance. The detection of anti-PEG abs directly from diluted blood serum was achieved within 40 min. Detection sensitivity is as good as or better than enzyme-linked immunosorbent assay (ELISA). Furthermore, different antibody isotypes can be quantitatively differentiated by adopting secondary antibodies. A pilot study has been performed to analyze clinical blood samples using this technology, demonstrating its potential as a convenient and powerful method to prescreen and monitor anti-PEG abs in the patients before or after they receive treatment with PEG-containing drugs.","is_dataset_classified":null,"base_score":3.5553480614894135,"endowment":3.5553480614894135,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"28727918","pmcid":"PMC5750323","openalex_id":"https://openalex.org/W2738773938","authors":[],"funders":[{"funder_name":"National Institute of Biomedical Imaging and Bioengineering","grant_id":"1 R21 EB020781-01","title":null},{"funder_name":"Defense Threat Reduction Agency","grant_id":"HDTRA1-13-1-0044","title":null},{"funder_name":"Division of Chemical, Bioengineering, Environmental, and Transport Systems","grant_id":"CBET-1264470","title":null},{"funder_name":"NIBIB NIH HHS","grant_id":"R21 EB020781","title":null}],"total_grants":4,"fwci":2.1254,"citation_percentile":0.86806505,"influential_citations":0,"citation_trend":[{"year":2018,"count":4},{"year":2019,"count":4},{"year":2020,"count":3},{"year":2021,"count":4},{"year":2022,"count":2},{"year":2023,"count":4},{"year":2024,"count":1},{"year":2025,"count":10},{"year":2026,"count":2}],"oa_status":"bronze","license":null,"oa_locations":[{"url":"https://pubs.acs.org/doi/pdf/10.1021/acs.analchem.7b02447?ref=article_openPDF","host_type":"journal"},{"url":"https://pubs.acs.org/doi/pdf/10.1021/acs.analchem.7b02447?ref=article_openPDF","host_type":"publisher"},{"url":"https://pubs.acs.org/doi/pdf/10.1021/acs.analchem.7b02447","host_type":"publisher"},{"url":"https://doi.org/10.1021/acs.analchem.7b02447","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/28727918","host_type":"repository"},{"url":"http://europepmc.org/pmc/articles/PMC5750323","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/5750323","host_type":"repository"}],"fields_of_study":["Monoclonal and Polyclonal Antibodies Research","Protein purification and stability","Advanced Biosensing Techniques and Applications","Animals","Antibodies","Biosensing Techniques","Humans","Limit of Detection","Methacrylates","Pilot Projects","Polyethylene Glycols","Rats","Surface Plasmon Resonance"],"mesh_terms":["Animals","Antibodies","Humans","Methacrylates","Pilot Projects","Polyethylene Glycols","Biosensing Techniques","Surface Plasmon Resonance","Rats","Limit of Detection"],"keywords":["PEG ratio","Ethylene glycol","Surface plasmon resonance","Chemistry","Antibody","Chromatography","Biosensor","Nanotechnology","Biochemistry","Nanoparticle","Materials science","Organic chemistry","Immunology"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-12T05:43:29.655742Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}