{"doi":"10.1021/acs.analchem.2c03089","title":"3-Acetylpyridine On-Tissue Paternò–Büchi Derivatization Enabling High Coverage Lipid C═C Location-Resolved MS Imaging in Biological Tissues","abstract":null,"journal":"Analytical Chemistry","year":2022,"id":603865,"datarank":0.5289540786924243,"base_score":3.5263605246161616,"endowment":3.5263605246161616,"self_citation_contribution":0.5289540786924243,"citation_network_contribution":0.0,"self_endowment_contribution":0.5289540786924243,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":33,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1549175,"name":"Chengyi Xie","orcid":null,"position":1,"is_corresponding":false},{"id":610990,"name":"Xiaoxiao Wang","orcid":"0000-0002-3996-7900","position":2,"is_corresponding":false},{"id":643006,"name":"Guodong Cao","orcid":"0000-0003-2854-8951","position":3,"is_corresponding":false},{"id":1549176,"name":"Yi Ru","orcid":null,"position":4,"is_corresponding":false},{"id":59026,"name":"Yuanyuan Song","orcid":"0000-0002-9183-5884","position":5,"is_corresponding":false},{"id":1549180,"name":"Ashok Iyaswamy","orcid":"0000-0003-0186-3863","position":6,"is_corresponding":false},{"id":896453,"name":"Min Li","orcid":"0000-0001-8803-3162","position":7,"is_corresponding":false},{"id":531938,"name":"Jianing Wang","orcid":"0000-0002-9294-2809","position":8,"is_corresponding":false},{"id":699574,"name":"Zongwei Cai","orcid":"0000-0002-8724-7684","position":9,"is_corresponding":false},{"id":1085070,"name":"Yanyan Chen","orcid":"0000-0002-7068-4669","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"3-Acetylpyridine On-Tissue Paternò–Büchi Derivatization Enabling High Coverage Lipid C═C Location-Resolved MS Imaging in Biological Tissues","abstract":"Unsaturated lipids containing single or more carbon-carbon double bonds (C═C) within tissues are closely associated with various types of diseases. Mass spectrometry imaging (MSI) has been used to study the spatial distribution of lipid C═C location isomers in tissue sections. However, comprehensive characterization of lipid C═C location isomers using MSI remains challenging. Herein, we established an on-tissue charge-switching Paternò-Büchi (PB) derivatization method using 3-acetylpyridine (3-AP) as a reaction reagent, which can be used to detect and assign C═C location of glycerophospholipids (GPLs) as well as neutral lipids, such as fatty acids (FAs), under the same experimental workflow using matrix-assisted laser desorption/ionization (MALDI)-MSI. High coverage of mono- and poly-unsaturated C═C location isomers among various lipid classes including FA, phosphatidylcholine (PC), and sulfatide (SHexCer) in distinct regions of the mouse brain and kidney was visualized using MALDI-MS/MS imaging. This method has also been applied to map the spatial distribution of lipid C═C location isomers in the Alzheimer's disease (AD) mice model for the first time, which provides a new tool to study the relationships between the distribution of lipid structural diversity and neurodegenerative diseases.","is_dataset_classified":null,"base_score":3.5263605246161616,"endowment":3.5263605246161616,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"36286543","pmcid":null,"openalex_id":"https://openalex.org/W4307431397","authors":[],"funders":[{"funder_name":"Health and Medical Research Fund","grant_id":"HMRF/17182541","title":null},{"funder_name":"Health and Medical Research Fund","grant_id":"HMRF/17182551","title":null},{"funder_name":"Health and Medical Research Fund","grant_id":"HMRF/19200721","title":null},{"funder_name":"Hong Kong Baptist University","grant_id":"HKBU-SKLP_2021_P05","title":null},{"funder_name":"Hong Kong Baptist University","grant_id":"HKBU/RC-IRCs/1718/03","title":null},{"funder_name":"Hong Kong Baptist University","grant_id":"IRCMS/19-20/H02","title":null},{"funder_name":"Hong Kong Baptist University","grant_id":"SKLP_2021_P04","title":null},{"funder_name":"Science, Technology and Innovation Commission of Shenzhen Municipality","grant_id":"2021Szvup134","title":null}],"total_grants":8,"fwci":2.6021,"citation_percentile":0.91019032,"influential_citations":0,"citation_trend":[{"year":2023,"count":8},{"year":2024,"count":14},{"year":2025,"count":9},{"year":2026,"count":2}],"oa_status":"closed","license":"https://doi.org/10.15223/policy-029","oa_locations":[{"url":"https://pubs.acs.org/doi/pdf/10.1021/acs.analchem.2c03089","host_type":"publisher"},{"url":"https://doi.org/10.1021/acs.analchem.2c03089","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/36286543","host_type":"repository"}],"fields_of_study":["Metabolomics and Mass Spectrometry Studies","Mass Spectrometry Techniques and Applications","Cancer, Lipids, and Metabolism"],"mesh_terms":["Animals","Carbon","Pyridines","Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization","Glycerophospholipids","Mice","Tandem Mass Spectrometry"],"keywords":["Chemistry","Mass spectrometry imaging","Derivatization","Phosphatidylcholine","Glycerophospholipids","Mass spectrometry","Reagent","Lipidomics","Matrix-assisted laser desorption/ionization","Phospholipid","Chromatography","Biochemistry","Desorption","Organic chemistry"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Clean water and sanitation"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-29T22:52:37.674324Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}