{"doi":"10.1021/acs.analchem.2c01221","title":"Rapid and Sensitive Detection of Antigen from SARS-CoV-2 Variants of Concern by a Multivalent Minibinder-Functionalized Nanomechanical Sensor","abstract":"New platforms for the rapid and sensitive detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants of concern are urgently needed. Here we report the development of a nanomechanical sensor based on the deflection of a microcantilever capable of detecting the SARS-CoV-2 spike (S) glycoprotein antigen using computationally designed multivalent minibinders immobilized on a microcantilever surface. The sensor exhibits rapid (<5 min) detection of the target antigens down to concentrations of 0.05 ng/mL (362 fM) and is more than an order of magnitude more sensitive than an antibody-based cantilever sensor. Validation of the sensor with clinical samples from 33 patients, including 9 patients infected with the Omicron (BA.1) variant observed detection of antigen from nasopharyngeal swabs with cycle threshold (Ct) values as high as 39, suggesting a limit of detection similar to that of the quantitative reverse transcription polymerase chain reaction (RT-qPCR). Our findings demonstrate the use of minibinders and nanomechanical sensors for the rapid and sensitive detection of SARS-CoV-2 and potentially other disease markers.","journal":"Analytical Chemistry","year":2022,"id":280772,"datarank":0.3453877639491069,"base_score":2.302585092994046,"endowment":2.302585092994046,"self_citation_contribution":0.3453877639491069,"citation_network_contribution":0.0,"self_endowment_contribution":0.3453877639491069,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":9,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9568,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":242358,"name":"Andrew C. Hunt","orcid":"0000-0001-9620-593X","position":1,"is_corresponding":false},{"id":955233,"name":"Gajendra S. Shekhawat","orcid":"0000-0003-3497-288X","position":2,"is_corresponding":false},{"id":108913,"name":"Lauren Carter","orcid":"0000-0002-9837-9068","position":3,"is_corresponding":false},{"id":853557,"name":"Sidney Chan","orcid":"0000-0002-1617-0464","position":4,"is_corresponding":false},{"id":805630,"name":"Kejia Wu","orcid":"0000-0002-4532-4290","position":5,"is_corresponding":false},{"id":218557,"name":"Longxing Cao","orcid":"0000-0003-4002-3648","position":6,"is_corresponding":false},{"id":105706,"name":"David Baker","orcid":"0000-0001-7896-6217","position":7,"is_corresponding":false},{"id":283126,"name":"Ramón Lorenzo-Redondo","orcid":"0000-0002-5462-9483","position":8,"is_corresponding":false},{"id":283133,"name":"Egon A. Ozer","orcid":"0000-0002-7131-3691","position":9,"is_corresponding":false},{"id":281228,"name":"Lacy M. Simons","orcid":"0000-0003-4041-6775","position":10,"is_corresponding":false},{"id":36741,"name":"Judd F. Hultquist","orcid":"0000-0001-6424-4280","position":11,"is_corresponding":false},{"id":242369,"name":"Michael C. Jewett","orcid":"0000-0003-2948-6211","position":12,"is_corresponding":false},{"id":461397,"name":"Vinayak P. Dravid","orcid":"0000-0002-6007-3063","position":13,"is_corresponding":false},{"id":955232,"name":"Dilip Agarwal","orcid":"0000-0002-7330-1491","position":0,"is_corresponding":true}],"reference_count":28,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T00:29:03.247347Z","pmid":"35652578","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}