{"doi":"10.1021/acs.analchem.2c00554","title":"Aptamer Sandwich Lateral Flow Assay (AptaFlow) for Antibody-Free SARS-CoV-2 Detection","abstract":"The COVID-19 pandemic is among the greatest health and socioeconomic crises in recent history. Although COVID-19 vaccines are being distributed, there remains a need for rapid testing to limit viral spread from infected individuals. We previously identified the SARS-CoV-2 spike protein N-terminal domain (NTD) binding DNA aptamer 1 (SNAP1) for detection of SARS-CoV-2 virus by aptamer–antibody sandwich enzyme-linked immunoassay (ELISA) and lateral flow assay (LFA). In this work, we identify a new aptamer that also binds at the NTD, named SARS-CoV-2 spike protein NTD-binding DNA aptamer 4 (SNAP4). SNAP4 binds with high affinity (<30 nM) for the SARS-CoV-2 spike protein, a 2-fold improvement over SNAP1. Furthermore, we utilized both SNAP1 and SNAP4 in an aptamer sandwich LFA (AptaFlow), which detected SARS-CoV-2 UV-inactivated virus at concentrations as low as 106 copies/mL. AptaFlow costs <$1 per test to produce, provides results in <1 h, and detects SARS-CoV-2 at concentrations that indicate higher viral loads and a high probability of contagious transmission. AptaFlow is a potential approach for a low-cost, convenient antigen test to aid the control of the COVID-19 pandemic.","journal":"Analytical Chemistry","year":2022,"id":236961,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":64,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9548,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":635773,"name":"Nataly Kacherovsky","orcid":null,"position":1,"is_corresponding":false},{"id":683854,"name":"Nuttada Panpradist","orcid":"0000-0002-2733-4110","position":2,"is_corresponding":false},{"id":858123,"name":"Ruixuan Wan","orcid":"0000-0001-6135-3166","position":3,"is_corresponding":false},{"id":858124,"name":"Joey Liang","orcid":"0000-0002-8510-3839","position":4,"is_corresponding":false},{"id":858125,"name":"Bo Zhang","orcid":"0000-0002-1737-1241","position":5,"is_corresponding":false},{"id":424556,"name":"Stephen J. Salipante","orcid":"0000-0001-8355-6992","position":6,"is_corresponding":false},{"id":233083,"name":"Barry R. Lutz","orcid":"0000-0003-4298-4901","position":7,"is_corresponding":false},{"id":19358,"name":"Suzie H. Pun","orcid":"0000-0003-1443-4996","position":8,"is_corresponding":false},{"id":685691,"name":"Lucy F. Yang","orcid":null,"position":0,"is_corresponding":true}],"reference_count":40,"raw_metadata":null,"created_at":"2026-07-19T00:22:03.686498Z","pmid":"35532905","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}