{"doi":"10.1021/acs.analchem.1c04321","title":"ER-GCaMP6f: An Endoplasmic Reticulum-Targeted Genetic Probe to Measure Calcium Activity in Astrocytic Processes","abstract":null,"journal":"Analytical Chemistry","year":2022,"id":655508,"datarank":0.4636563680037475,"base_score":3.091042453358316,"endowment":3.091042453358316,"self_citation_contribution":0.4636563680037475,"citation_network_contribution":0.0,"self_endowment_contribution":0.4636563680037475,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":21,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":459144,"name":"Mengfan Xia","orcid":"0009-0007-6253-0049","position":1,"is_corresponding":false},{"id":586516,"name":"Ebubechi Adindu","orcid":null,"position":2,"is_corresponding":false},{"id":597234,"name":"Cooper Riley Davis","orcid":"0000-0003-3175-8036","position":3,"is_corresponding":false},{"id":502969,"name":"Pavel I. Ortinski","orcid":"0000-0003-0814-4490","position":4,"is_corresponding":false},{"id":286545,"name":"Christopher I. Richards","orcid":"0000-0003-0019-1989","position":5,"is_corresponding":false},{"id":388653,"name":"Surya P. Aryal","orcid":"0000-0002-7513-4092","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"ER-GCaMP6f: An Endoplasmic Reticulum-Targeted Genetic Probe to Measure Calcium Activity in Astrocytic Processes","abstract":"Ca 2+ is a major second messenger involved in cellular and subcellular signaling in a wide range of cells, including astrocytes, which use calcium ions to communicate with other cells in the brain. Even though a variety of genetically encoded Ca 2+ indicators have been developed to study astrocyte calcium signaling, understanding the dynamics of endoplasmic reticulum calcium signaling is greatly limited by the currently available tools. To address this, we developed an endoplasmic reticulum-targeted calcium indicator, ER-GCaMP6f, which is anchored to the cytosolic side of the organelle and measures signaling that occurs in close proximity to the endoplasmic reticulum of astrocytes. Using a combination of confocal and super-resolution microscopy techniques, we demonstrate the localization of the indicator in the endoplasmic reticulum in both cell soma and processes of astrocytes. Combining ER-GCaMP6f with total internal reflection fluorescence microscopy, we show that Ca 2+ fluctuations in small astrocytic processes can be detected, which are otherwise not observable with existing indicators and standard wide-field and confocal techniques. We also compared the ER-GCaMP6f indicator against currently used plasma membrane-tethered and cytosolic GCaMP6f indicators. ER-GCaMP6f identifies dynamics in calcium signaling of endoplasmic reticulum resident receptors that are missed by plasma membrane-anchored indicators. We also generated an adeno-associated virus (AAV5) and demonstrate that ER-GCaMP6f can be expressed in vivo and by measured calcium activity in brain slices. ER-GCaMP6f provides a powerful tool to study calcium signaling in close proximity to the endoplasmic reticulum in astrocyte cell soma and processes both in culture and in brain slices.","is_dataset_classified":null,"base_score":3.091042453358316,"endowment":3.091042453358316,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"35061939","pmcid":"PMC9047445","openalex_id":"https://openalex.org/W4206985743","authors":[],"funders":[{"funder_name":"Foundation for the National Institutes of Health","grant_id":"DA038817","title":null},{"funder_name":"Foundation for the National Institutes of Health","grant_id":"R01 DA041513","title":null},{"funder_name":"NIDA NIH HHS","grant_id":"R01 DA053070","title":null},{"funder_name":"NIDA NIH HHS","grant_id":"R21 DA038817","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM138837","title":null},{"funder_name":"NIDA NIH HHS","grant_id":"R33 DA038817","title":null},{"funder_name":"National Institutes of Health","grant_id":"5R01DA041513-05","title":"Neural microcircuit selection by astrocyte signaling following cocaine exposure"}],"total_grants":7,"fwci":2.1536,"citation_percentile":0.86648527,"influential_citations":0,"citation_trend":[{"year":2022,"count":2},{"year":2023,"count":4},{"year":2024,"count":6},{"year":2025,"count":8},{"year":2026,"count":1}],"oa_status":"green","license":"STM Policy #29","oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/9047445","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/9047445","host_type":"repository"},{"url":"https://pubs.acs.org/doi/pdf/10.1021/acs.analchem.1c04321","host_type":"publisher"},{"url":"https://doi.org/10.1021/acs.analchem.1c04321","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/35061939","host_type":"repository"}],"fields_of_study":["Cellular transport and secretion","Retinal Development and Disorders","Lipid Membrane Structure and Behavior","0301 basic medicine","0303 health sciences","03 medical and health sciences"],"mesh_terms":["Astrocytes","Calcium","Cytosol","Endoplasmic Reticulum","Calcium Signaling"],"keywords":["Endoplasmic reticulum","Calcium signaling","Cell biology","STIM1","Calcium","Organelle","Chemistry","Membrane contact site","Cytosol","Signal transduction","Biochemistry","Biology","Membrane","Membrane protein","Integral membrane protein","Astrocytes"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-11T13:44:00.536606Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}