{"doi":"10.1021/ac950914h","title":"Mass Spectrometric Sequencing of Proteins from Silver-Stained Polyacrylamide Gels","abstract":"Proteins from silver-stained gels can be digested enzymatically and the resulting peptide analyzed and sequenced by mass spectrometry. Standard proteins yield the same peptide maps when extracted from Coomassie- and silver-stained gels, as judged by electrospray and MALDI mass spectrometry. The low nanogram range can be reached by the protocols described here, and the method is robust. A silver-stained one-dimensional gel of a fraction from yeast proteins was analyzed by nano-electrospray tandem mass spectrometry. In the sequencing, more than 1000 amino acids were covered, resulting in no evidence of chemical modifications due to the silver staining procedure. Silver staining allows a substantial shortening of sample preparation time and may, therefore, be preferable over Coomassie staining. This work removes a major obstacle to the low-level sequence analysis of proteins separated on polyacrylamide gels.","journal":"Analytical Chemistry","year":1996,"id":1085,"datarank":19.959276696557016,"base_score":9.111293218374948,"endowment":9.111293218374948,"self_citation_contribution":1.3666939827562425,"citation_network_contribution":18.592582713800773,"self_endowment_contribution":1.3666939827562425,"citer_contribution":18.592582713800773,"corpus_percentile":94.2,"corpus_rank":1307,"citation_count":9056,"citer_count":154,"citers_with_citation_signal":154,"citers_with_endowment":154,"datacite_reuse_total":0,"is_dataset":false,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"1996-03-01","authors":[{"id":14858,"name":"Matthias Wilm","orcid":"0000-0002-5461-6834","position":1,"is_corresponding":false},{"id":14859,"name":"Ole Vorm","orcid":null,"position":2,"is_corresponding":false},{"id":2937,"name":"Matthias Mann","orcid":"0000-0003-1292-4799","position":3,"is_corresponding":false},{"id":14857,"name":"Andrej Shevchenko","orcid":"0000-0002-5079-1109","position":0,"is_corresponding":true}],"reference_count":41,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-03-01T18:20:47.508186Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}