{"doi":"10.1021/ac9021083","title":"Size-Sorting Combined with Improved Nanocapillary Liquid Chromatography−Mass Spectrometry for Identification of Intact Proteins up to 80 kDa","abstract":null,"journal":"Analytical Chemistry","year":2010,"id":611297,"datarank":0.6892679775201885,"base_score":4.59511985013459,"endowment":4.59511985013459,"self_citation_contribution":0.6892679775201885,"citation_network_contribution":0.0,"self_endowment_contribution":0.6892679775201885,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":98,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1573181,"name":"John C. 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Comparative work on protein standards showed that a polymeric stationary phase led to superior sensitivity over a silica-based medium in reversed-phase nanocapillary LC, with detection of proteins >50 kDa routinely accomplished in the linear ion trap of a hybrid Fourier transform mass spectrometer. Protein identification was enabled by nozzle-skimmer dissociation and detection of fragment ions with <10 ppm mass accuracy for highly specific database searching using tailored software. This overall approach led to identification of proteins up to 80 kDa, with 10-60 proteins identified in single LC-MS runs of samples from yeast and human cell lines prefractionated by their molecular mass using a gel-based sieving system.","is_dataset_classified":null,"base_score":4.59511985013459,"endowment":4.59511985013459,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"20073486","pmcid":"PMC2823583","openalex_id":"https://openalex.org/W148173047","authors":[],"funders":[{"funder_name":"NIGMS NIH HHS","grant_id":"GM 067193-07","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM067193","title":null}],"total_grants":2,"fwci":6.7196,"citation_percentile":0.97849187,"influential_citations":0,"citation_trend":[{"year":2012,"count":10},{"year":2013,"count":8},{"year":2014,"count":11},{"year":2015,"count":4},{"year":2016,"count":9},{"year":2017,"count":7},{"year":2018,"count":2},{"year":2019,"count":2},{"year":2020,"count":5},{"year":2021,"count":2},{"year":2022,"count":3},{"year":2023,"count":3},{"year":2024,"count":3},{"year":2026,"count":1}],"oa_status":"green","license":null,"oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2823583","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2823583","host_type":"repository"},{"url":"https://pubs.acs.org/doi/pdf/10.1021/ac9021083","host_type":"publisher"},{"url":"https://doi.org/10.1021/ac9021083","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/20073486","host_type":"repository"}],"fields_of_study":["Mass Spectrometry Techniques and Applications","Advanced Proteomics Techniques and Applications","Metabolomics and Mass Spectrometry Studies","Amino Acid Sequence","Animals","Cattle","Chromatography, Liquid","HeLa Cells","Humans","Mass Spectrometry","Molecular Sequence Data","Molecular Weight","Nanotechnology","Polymers","Porosity","Proteins","Proteome","Saccharomyces cerevisiae Proteins","Time Factors"],"mesh_terms":["Amino Acid Sequence","Animals","Cattle","Chromatography, Liquid","HeLa Cells","Humans","Molecular Sequence Data","Molecular Weight","Polymers","Proteins","Mass Spectrometry","Time Factors","Porosity","Proteome","Saccharomyces cerevisiae Proteins","Nanotechnology","Hela Cells"],"keywords":["Chemistry","Mass spectrometry","Chromatography","Top-down proteomics","Proteomics","Proteome","Bottom-up proteomics","Ion trap","Tandem mass spectrometry","Quadrupole ion trap","Analytical Chemistry (journal)","Gel electrophoresis","Resolution (logic)","Protein mass spectrometry","Biochemistry"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"uniprot"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-01T17:58:57.664870Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}