{"doi":"10.1021/ac3018845","title":"Detection of Pathogenic Bacteria by Using Zinc Finger Protein Fused with Firefly Luciferase","abstract":null,"journal":"Analytical Chemistry","year":2012,"id":630975,"datarank":0.4887144807032224,"base_score":3.258096538021482,"endowment":3.258096538021482,"self_citation_contribution":0.4887144807032224,"citation_network_contribution":0.0,"self_endowment_contribution":0.4887144807032224,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":25,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1634927,"name":"Takenori Kumagai","orcid":null,"position":1,"is_corresponding":false},{"id":404688,"name":"Chiaki Takahashi","orcid":"0000-0003-3390-9563","position":2,"is_corresponding":false},{"id":1634928,"name":"Aki Kezuka","orcid":null,"position":3,"is_corresponding":false},{"id":1634930,"name":"Yoshiyuki Murakami","orcid":null,"position":4,"is_corresponding":false},{"id":1634931,"name":"Yuko Osawa","orcid":null,"position":5,"is_corresponding":false},{"id":1634932,"name":"Hiroaki Motoki","orcid":null,"position":6,"is_corresponding":false},{"id":1634933,"name":"Takafumi Matsuo","orcid":null,"position":7,"is_corresponding":false},{"id":1634934,"name":"Michio Horiuchi","orcid":null,"position":8,"is_corresponding":false},{"id":210319,"name":"Koji Sode","orcid":null,"position":9,"is_corresponding":false},{"id":1634935,"name":"Shizunobu Igimi","orcid":null,"position":10,"is_corresponding":false},{"id":112386,"name":"Kazunori Ikebukuro","orcid":null,"position":11,"is_corresponding":false},{"id":112384,"name":"Koichi Abe","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Detection of Pathogenic Bacteria by Using Zinc Finger Protein Fused with Firefly Luciferase","abstract":"We constructed a novel bacterial genome detection system using zinc finger protein (ZF) fused with firefly luciferase (ZF-luciferase). Taking advantage of the direct recognition of double-stranded DNA (dsDNA) by ZF, we previously constructed bacterial genome detection systems that did not require dehybridization processes. To detect polymerase chain reaction (PCR) products rapidly and with a high sensitivity, we constructed two kinds of ZF-luciferase, Sp1-fused luciferase (Sp1-luciferase), and Zif268-fused luciferase (Zif268-luciferase). ZF-luciferase not only maintains luciferase activity but also shows dsDNA-binding ability and specificity. Furthermore, we succeeded in the detection of 10 copies of the genome of Legionella pneumophila and Escherichia coli O157. ZF-luciferase would be a useful tool for highly sensitive detection of pathogenic bacterial genome.","is_dataset_classified":null,"base_score":3.258096538021482,"endowment":3.258096538021482,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"22894566","pmcid":null,"openalex_id":"https://openalex.org/W2333796273","authors":[],"funders":[],"total_grants":0,"fwci":1.6002,"citation_percentile":0.82012809,"influential_citations":0,"citation_trend":[{"year":2012,"count":1},{"year":2013,"count":3},{"year":2014,"count":1},{"year":2015,"count":3},{"year":2016,"count":4},{"year":2017,"count":2},{"year":2018,"count":3},{"year":2019,"count":1},{"year":2020,"count":2},{"year":2021,"count":3},{"year":2022,"count":1},{"year":2026,"count":1}],"oa_status":"closed","license":null,"oa_locations":[{"url":"https://pubs.acs.org/doi/pdf/10.1021/ac3018845","host_type":"publisher"},{"url":"https://doi.org/10.1021/ac3018845","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/22894566","host_type":"repository"}],"fields_of_study":["Vibrio bacteria research studies","Legionella and Acanthamoeba research","Biosensors and Analytical Detection","Binding Sites","DNA, Bacterial","Early Growth Response Protein 1","Escherichia coli","Legionella pneumophila","Luciferases, Firefly","Polymerase Chain Reaction","Recombinant Fusion Proteins","Sp1 Transcription Factor","Zinc Fingers"],"mesh_terms":["Binding Sites","DNA, Bacterial","Escherichia coli","Recombinant Fusion Proteins","Polymerase Chain Reaction","Sp1 Transcription Factor","Zinc Fingers","Legionella pneumophila","Luciferases, Firefly","Early Growth Response Protein 1"],"keywords":["Luciferase","Chemistry","Escherichia coli","Molecular biology","Biochemistry","Gene","Biology","Transfection"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-05T22:33:31.820540Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}