{"doi":"10.1017/s0266467425100102","title":"Population genetics of the endangered black walnut <i>Juglans neotropica</i> (Juglandaceae) based on plastid data from the Amazonas region","abstract":"<jats:title>Abstract</jats:title>\n\t  <jats:p>The black walnut <jats:italic>Juglans neotropica</jats:italic> is a forest species characterized by being a monoecious and deciduous tree with a long life. This species has great ecological, environmental, and economic value, playing a fundamental role in the ecosystem. According to the IUCN, <jats:italic>J. neotropica</jats:italic> is threatened by anthropogenic activities that have drastically affected its distribution. In this study, the plastid intergenic spacer marker <jats:italic>trnS-trnfM</jats:italic> was amplified from 74 <jats:italic>J. neotropica</jats:italic> samples from eight locations in Amazonas region (Peru) to determine its haplotype network, genetic diversity, and genetic divergence. The results revealed that <jats:italic>J. neotropica</jats:italic> from Amazonas region showed i) a lineage composed of the eight populations embedded into the Rhysocaryon section; ii) three genetic groups within the haplotype network with the presence of an ancestral haplotype (H1) and possibly candidates for new taxa; iii) a high divergence between the populations of Molinopampa and Luya (1.62–2.64% of p-distance); iv) populations with high genetic diversity indices (Levanto = 0.32, Molinopampa = 0.41) with constant threats from anthropogenic activities; and v) high genetic structuring within populations (Fst = 0.04). Overall, these results collectively support a scenario of high variability with limited interpopulation genetic exchange. Our findings provide previously unavailable insights into the vulnerability of the black walnut <jats:italic>J. neotropica</jats:italic> by (i) quantifying the genetic consequences of human-induced habitat fragmentation and (ii) establishing baseline diversity metrics for future monitoring. These results directly inform <jats:italic>in situ</jats:italic> conservation priorities by identifying populations harbouring unique alleles that warrant immediate protection. Finally, further research should include nuclear markers (e.g., microsatellites or RAD-seq) to support our findings.</jats:p>","journal":"Journal of Tropical Ecology","year":2025,"id":645337,"datarank":0.16479184330021646,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"self_citation_contribution":0.16479184330021646,"citation_network_contribution":0.0,"self_endowment_contribution":0.16479184330021646,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1680315,"name":"Samia L.J. Fernandez-Güimac","orcid":"0000-0001-5176-1545","position":1,"is_corresponding":false},{"id":1680316,"name":"Danilo E. Bustamante","orcid":"0000-0002-5979-6993","position":2,"is_corresponding":false},{"id":1680317,"name":"Martha S. Calderon","orcid":"0000-0003-3611-140X","position":3,"is_corresponding":false},{"id":1680314,"name":"Jessica C. Llaja","orcid":"0000-0002-0995-6186","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Population genetics of the endangered black walnut <i>Juglans neotropica</i> (Juglandaceae) based on plastid data from the Amazonas region","abstract":"<jats:title>Abstract</jats:title>\n\t  <jats:p>The black walnut <jats:italic>Juglans neotropica</jats:italic> is a forest species characterized by being a monoecious and deciduous tree with a long life. This species has great ecological, environmental, and economic value, playing a fundamental role in the ecosystem. According to the IUCN, <jats:italic>J. neotropica</jats:italic> is threatened by anthropogenic activities that have drastically affected its distribution. In this study, the plastid intergenic spacer marker <jats:italic>trnS-trnfM</jats:italic> was amplified from 74 <jats:italic>J. neotropica</jats:italic> samples from eight locations in Amazonas region (Peru) to determine its haplotype network, genetic diversity, and genetic divergence. The results revealed that <jats:italic>J. neotropica</jats:italic> from Amazonas region showed i) a lineage composed of the eight populations embedded into the Rhysocaryon section; ii) three genetic groups within the haplotype network with the presence of an ancestral haplotype (H1) and possibly candidates for new taxa; iii) a high divergence between the populations of Molinopampa and Luya (1.62–2.64% of p-distance); iv) populations with high genetic diversity indices (Levanto = 0.32, Molinopampa = 0.41) with constant threats from anthropogenic activities; and v) high genetic structuring within populations (Fst = 0.04). Overall, these results collectively support a scenario of high variability with limited interpopulation genetic exchange. Our findings provide previously unavailable insights into the vulnerability of the black walnut <jats:italic>J. neotropica</jats:italic> by (i) quantifying the genetic consequences of human-induced habitat fragmentation and (ii) establishing baseline diversity metrics for future monitoring. These results directly inform <jats:italic>in situ</jats:italic> conservation priorities by identifying populations harbouring unique alleles that warrant immediate protection. Finally, further research should include nuclear markers (e.g., microsatellites or RAD-seq) to support our findings.</jats:p>","is_dataset_classified":null,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"19910364","pmcid":null,"openalex_id":"https://openalex.org/W4411358049","authors":[],"funders":[],"total_grants":0,"fwci":1.233,"citation_percentile":0.77347759,"influential_citations":0,"citation_trend":[{"year":2026,"count":2}],"oa_status":"hybrid","license":"cc-by","oa_locations":[{"url":"https://www.cambridge.org/core/services/aop-cambridge-core/content/view/5B5672D5CF4159BB683D9A3C8B2E4513/S0266467425100102a.pdf/div-class-title-population-genetics-of-the-endangered-black-walnut-span-class-italic-juglans-neotropica-span-juglandaceae-based-on-plastid-data-from-the-amazonas-region-div.pdf","host_type":"journal"},{"url":"https://www.cambridge.org/core/services/aop-cambridge-core/content/view/5B5672D5CF4159BB683D9A3C8B2E4513/S0266467425100102a.pdf/div-class-title-population-genetics-of-the-endangered-black-walnut-span-class-italic-juglans-neotropica-span-juglandaceae-based-on-plastid-data-from-the-amazonas-region-div.pdf","host_type":"publisher"},{"url":"https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0266467425100102","host_type":"publisher"},{"url":"https://doi.org/10.1017/s0266467425100102","host_type":"journal"}],"fields_of_study":["Nuts composition and effects","Plant and animal studies","Genetic diversity and population structure"],"mesh_terms":[],"keywords":["Juglandaceae","Juglans","Endangered species","Plastid","Population genetics","Population","Geography","Biology","Botany","Genetics","Medicine","Chloroplast","Gene","Environmental health"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-09T04:55:34.323901Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}